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Denaturation map of the ribosomal DNA ofLytechinus variegatus sperm

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Summary

Electron microscopy of the partially heat denatured ribosomal DNA (rDNA) from sea urchin (Lytechinus variegatus) sperm has demonstrated that it consists of repeating units of 3.6±0.2 μm, corresponding to a mol.wt of 7.2±0.4×106. Based on differential denaturability, each repeat unit is divided into 2 regions. The larger region of 2.47±0.11 μm (mol.wt 4.9±0.22×106) corresponds in length to the ribosomal precursor RNA of sea urchins and the smaller, GC-rich, subunit of 1.16±0.09 μm (mol.wt 2.3±0.18×106) is presumed to contain non-transcribed spacer sequences.

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Author notes

  1. N. K. Mishra

    Present address: Department of Zoology, Patna University, 80005, Patna, Bihr, India

Authors and Affiliations

  1. Department of Zoology, University of North Carolina, Chapel Hill, N.C., USA

    N. K. Mishra

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  1. N. K. Mishra
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Acknowledgments. This paper is dedicated to the memory of Dr D. P. Costello whose insprration made this work possible. I gratefully acknowledge the valuable advice of Drs D.W. Stafford and M.A. Bleyman.

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Mishra, N.K. Denaturation map of the ribosomal DNA ofLytechinus variegatus sperm. Experientia 35, 1161–1163 (1979). https://doi.org/10.1007/BF01963262

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