Abstract
Two new monoclonal antibodies (MN14G21.17 and MN14E9.15) specific for the serotype 4 antigen of meningococci were isolated. The antibodies were raised against a previously non-typable serogroup B strain from the Netherlands and were shown to react with the serotype antigen of the prototype reference strains for serotype 4 and serogroup A as well as with that of the homologous strain. Further screening of 290 serogroup A and B case isolates with the monoclonal antibodies indicated that the serotype 4 epitope was present on all 100 serogroup A strains tested, including representative isolates from 28 epidemics, and on most isolates from a recent serogroup B epidemic (1981–1983) in Cuba. In addition, 68 isolates from recent sporadic cases (1980–1986) and/or clusters of cases of serogroup B disease in many parts of Europe, the USA, the USSR and Australia were also of this serotype.
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References
Peltola, H. Meningococcal disease: still with us. Reviews of Infectious Diseases 1983, 5: 71–91.
Branham, S. E. Serological relationships among meningococci. Bacteriological Reviews 1983, 17: 175–188.
Poolman, J. T., Jonsdottir, K., Jones, D. M., Lind, I., Froholm, H. C., Zanen, H. C. Meningococcal serotypes and serogroup B disease in North-West Europe. Lancet 1986, ii: 555–557.
Frasch, C. E., Zollinger, W. D., Poolman, J. T. A proposed nomenclature for designation of serotypes withinNeisseria meningitidis. Reviews of Infectious Diseases 1985, 7: 504–508.
Poolman, J. T., Buchanan, T. M. Monoclonal antibodies against meningococcal outer membrane proteins. Medicine Tropicale 1983, 43: 139–142.
Abdillahi, H., Poolman, J. T., Hopman, C. T. P., Zanen, H. C. Sero- and subtyping of non-2a, non-2b, and non-15 group BNeisseria meningitidis. Antonie van Leewenhoek 1986, 52: 242–244.
Olyhoek, T., Crowe, B. A., Achtman, M. Epidemiological analysis and geographical distribution ofNeisseria meningitidis group A. In: Schoolnik, G. (ed.): The pathogenicNeisseria. American Society for Microbiology, Washington, DC, 1985, p. 530–535.
Olyhoek, T., Crowe, B. A., Achtman, M. Evidence for clonal structure inNeisseria meningitidis: relevance to epidemiology. Antonie van Leewenhoek 1986, 52: 202–205.
Kohler, G., Milstein, C. Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature 1976, 256: 495–497.
Poolman, J. T., Buchanan, T. M. Monoclonal antibodies activity against native and denatured forms of gonococcal outer membrane proteins detected with ultrathin, longitudinal slices of polyacrylamide gels. Journal of Immunological Methods 1984, 75: 265–274.
Poolman, J. T., Abdillahi, H. Outer membrane protein serosubtyping ofNeisseria meningitidis. European Journal of Clinical Microbiology and Infectious Diseases 1988, 7: 291–292.
Frasch, C. E., Chapman, S. S. Classification ofNeisseria meningitidis group B into distinct serotypes. III: Application of a new bactericidal-inhibition technique to distribution of serotypes among cases and carriers. Journal of Infectious Diseases 1973, 127: 149–154.
Sipple, J. E., Quan, A. Homogeneity of protein serotype antigens inNeisseria meningitidis group A. Infection and Immunity 1977, 16: 623–627.
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Abdillahi, H., Crowe, B.A., Achtman, M. et al. Two monoclonal antibodies specific for serotype 4 antigen ofNeisseria meningitidis . Eur. J. Clin. Microbiol. Infect. Dis. 7, 293–296 (1988). https://doi.org/10.1007/BF01963105
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DOI: https://doi.org/10.1007/BF01963105