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Enzyme immunoassay for the detection ofGiardia lamblia

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Abstract

An enzyme immunoassay (EIA) was developed for direct detection ofGiardia lamblia antigens in fecal specimens. The EIA was evaluated by testing specimens from 1,331 subjects in the USA and Egypt. For the 353 specimens from human subjects in the USA there was a 97% overall agreement between the results of the EIA and direct microscopic examination, yielding a sensitivity and specificity of 92% and 99% respectively. Due to adverse field conditions the EIA did not perform as well in the specimens collected and tested in Egypt. The sensitivity and specificity for 585 human specimens from Egypt were 74% and 97% respectively.

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Authors and Affiliations

  1. Department of Pathology, MS1A, School of Medicine, University of California, 95616, Davis, California, USA

    J. R. Carlson, D. J. Harry & M. A. Stork

  2. Program in Infectious Diseases and Clinical Microbiology, School of Medicine, University of Texas Health Science Center, Houston, Texas, USA

    P. S. Sullivan, S. A. Thorton & H. L. DuPont

Authors
  1. J. R. Carlson
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  2. P. S. Sullivan
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  3. D. J. Harry
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  4. M. A. Stork
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  5. S. A. Thorton
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  6. H. L. DuPont
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Carlson, J.R., Sullivan, P.S., Harry, D.J. et al. Enzyme immunoassay for the detection ofGiardia lamblia . Eur. J. Clin. Microbiol. Infect. Dis. 7, 538–540 (1988). https://doi.org/10.1007/BF01962610

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  • DOI: https://doi.org/10.1007/BF01962610

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