Summary
The transience of caffeine-induced tensions in crayfish muscle fibers indicates a caffeine-insensitive, Ca-transport system in these fibers. Analysis of the changes in relaxation time of caffeine tensions following ionic substitutions in the crayfish saline leads to the conclusions that this Ca-transport system is located in the sarcolemma and that the extrusion process involves binding of external cations, in particular Na.
Similar content being viewed by others
References
Bianchi, C. P. 1961. Effect of caffeine on radiocalcium movement in frog sartorius.J. Gen. Physiol. 44:845.
Blaustein, M. P., Hodgkin, A. L. 1969. The effect of cyanide on the efflux of Ca from squid axon.J. Physiol. 200:497.
Caputo, C. 1966. Caffeine- and potassium-induced contractures of frog striated muscle fibers in hypertonic solutions.J. Gen. Physiol. 50:129.
Chiarandini, D. J., Reuben, J. P., Brandt, P. W., Grundfest, H. 1970a. Effects of caffeine on crayfish muscle fibers. I. Activation of contration and induction of Ca spike electrogenesis.J. Gen. Physiol. 55:640.
——, Girardier, L., Katz, G. M., Grundfest, H. 1970b. Effects of caffeine on crayfish muscle fibers. II. Refractoriness and factors influencing recovery (reprimiing) of contractile responses.J. Gen. Physiol. 55:665.
Ebashi, S., Endo, M. 1968. Calcium ion and muscle contraction.Progr. Biophys. 18:123.
Frank, G. B. 1962. Utilization of bound Ca in the action of caffeine and certain multivalent cations on skeletal muscle.J. Physiol. 163:254.
Hagiwara, S., Nakajima, S. 1966. Differences in Na and Ca spikes as examined by application of tetrodotoxin, procaine and manganese ions.J. Gen. Physiol. 49:793.
Lee, K. S., Shin, B. C. 1969. Studies on the active transport of Ca in human red cells.J. Gen. Physiol. 54:713.
Lüttgau, H. C., Oetliker, M. 1968. The action of caffeine on the activation of the contraction mechanism in striated muscle fibers.J. Physiol. 194:51.
Niedergerke, R. 1963. Movements of Ca in frog heart vertricles at rest and during contractures.J. Physiol. 167:515.
Reuben, J. P., Brandt, P. W., Girardier, L., Grundfest, H. 1967a. Crayfish Muscle: Permeability to sodium induced by calcium depletion.Science 155:1263.
——, Garcia, H., Grundfest, H. 1967b. Excitation-contraction coupling in crayfish.Amer. Zool. 7:623.
Reuter, H., Seitz, N. 1968. The dependence of Ca efflux from cardiac muscle on temperatur and external ion composition.J. Physiol. 195:451.
Schatzmann, H. J. 1966. ATP-dependent Ca extrusion from human red cells.Experientia 22:364.
—, Vicenzi, F. F. 1969. Calcium movements across the membrane of human red cells.J. Gen. Physiol. 201:369.
Takeda, K. 1967. Permeability changes associated with the action potential in procainetreated crayfish abdominal muscle fibers.J. Gen. Physiol. 50:1049.
Weber, A., Herz, R. 1968. The relationship between caffeine contracture of intact muscle and the effect of caffeine on reticulum.J. Gen. Physiol. 52:750.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Orentlicher, M., Ornstein, R.S. Influence of external cations on caffeine-induced tension: Calcium extrusion in crayfish muscle. J. Membrain Biol. 5, 319–333 (1971). https://doi.org/10.1007/BF01957349
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01957349