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Effects of isoxazolyl-naphthoquinoneimines on growth and oxygen radical production inTrypanosoma cruzi andCrithidia fasciculata

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Summary

Several 4-(aminomethylisoxazolyl)-1,2-naphthoquinones inhibited growth and DNA synthesis inTrypanosoma cruzi and stimulated O2 uptake and\(O_2 ^{\bar .} \) generation by the parasite epimastigotes and their mitochondrial and microsomal membranes; these results support the idea that oxygen radicals play a role in quinone toxicity. Maximal effects on respiration and\(O_2 ^{\bar .} \) generation were observed with antimycin-inhibited cells. Similar results as well as stimulation of H2O2 production were obtained withCrithidia fasciculata despite the presence of catalase in this organism.

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Authors and Affiliations

  1. Bioenergetics Research Centre and Department of Biochemistry, School of Medicine, University of Buenos Aires, Paraguay 2155, 1121, Buenos Aires, Argentina

    M. N. Schwarcz de Tarlovsky, S. G. Goijman, M. P. Molina Portela & A. O. M. Stoppani

Authors
  1. M. N. Schwarcz de Tarlovsky
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  2. S. G. Goijman
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  3. M. P. Molina Portela
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  4. A. O. M. Stoppani
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Additional information

Acknowledgments. This work was aided by grants from the University of Buenos Aires, the Scientific Office of the American States Organization and CEDIQUIFA (Buenos Aires). S.G.G. and M.P.M.P. are Research Fellows and A.O.M.S. is Career Investigator of CONICET (Argentina). L. T. Viñas and M. G. Gutierrez lent able technical assistance.

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Schwarcz de Tarlovsky, M.N., Goijman, S.G., Molina Portela, M.P. et al. Effects of isoxazolyl-naphthoquinoneimines on growth and oxygen radical production inTrypanosoma cruzi andCrithidia fasciculata. Experientia 46, 502–505 (1990). https://doi.org/10.1007/BF01954247

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  • DOI: https://doi.org/10.1007/BF01954247

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