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Active transport of [32P]thiamine diphosphate inEscherichia coli

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Summary

Active uptake of [32P]thiamine diphosphate byE. coli was analyzed using an improved method of gel filtration chromatography. The radioactive coenzyme was accumulated without dephosphorylation. From this result it was concluded that thiamine kinase is not involved in the membrane transport of thiamine inE. coli.

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Literatur

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Author notes

  1. R. Hayashi

    Present address: Ohita Medical College, 879-56, Ohita, Japan

  2. T. Nishimune

    Present address: Yamaguchi University School of Medicine, Yamaguchi, Japan

Authors and Affiliations

  1. Department of Microbiology, Yamaguchi University School of Medicine, 755, Ube, Yamaguchi-ken, Japan

    T. Nishimune & R. Hayashi

  2. Ohita Medical College, 879-56, Ohita, Japan

    T. Nishimune

Authors
  1. T. Nishimune
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  2. R. Hayashi
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Additional information

We are indebted to Miss M. Abe for her technical assistance.

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Nishimune, T., Hayashi, R. Active transport of [32P]thiamine diphosphate inEscherichia coli. Experientia 36, 916–918 (1980). https://doi.org/10.1007/BF01953791

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  • DOI: https://doi.org/10.1007/BF01953791

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