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Quantitation of proinsulin mRNA sequences in hamster insulinoma cells in culture by molecular hybridization

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Summary

The content of proinsulin mRNA sequences was measured in a cultured cell line established from a transplantable hamster islet cell tumor, by hybridization with proinsulin cDNA. The cells cultured in vitro were found to contain a significant amount of proinsulin mRNA sequences, compared with non-insulin-producing hamster tissues, and seem to be a useful system for the study of insulin gene expression.

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Authors and Affiliations

  1. Department of Biochemistry, Toyama Medical and Pharmaceutical University School of Medicine, Toyama, 930-01, Toyama, Japan

    H. Yamamoto, K. Nose, N. Itoh & H. Okamoto

Authors
  1. H. Yamamoto
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  2. K. Nose
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  3. N. Itoh
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  4. H. Okamoto
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Additional information

This work was supported in part by Grants-in-Aid Scientific Research (248129, 387065, 337013, 378052) and a Grant-in-Aid for Cancer Research (301538) from the Ministry of Education, Science and Culture, Japan.

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Yamamoto, H., Nose, K., Itoh, N. et al. Quantitation of proinsulin mRNA sequences in hamster insulinoma cells in culture by molecular hybridization. Experientia 36, 187–188 (1980). https://doi.org/10.1007/BF01953722

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  • DOI: https://doi.org/10.1007/BF01953722

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