Abstract
The kinetics of insulin secretion, not just the total amount, is of decisive relevance for the physiological regulation of glucose homeostasis. Thus to characterize the relevant features of the secretory response to an insulinotropic stimulus a method is needed which is able to resolve the temporal response pattern, in particular to distinguish the first phase from the second phase response. The perifusion of collagenase-isolated islets is a method which permits to register responses of near-physiological complexity with a preparation that can also be used for cell physiological and biochemical investigations on stimulus-secretion oupling.
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Acknowledgment
Help by Verena Lier-Glaubitz, Michael Belz, and Hany Ghaly is gratefully acknowledged. Work in the authors´ lab was supported by grants from the Deutsche Forschungsgemeinschaft (DFG Ru 368/5-1), the Deutsche Diabetes Gesellschaft, and the Deutsche Diabetes Stiftung.
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Willenborg, M., Schumacher, K., Rustenbeck, I. (2012). Determination of Beta-Cell Function: Insulin Secretion of Isolated Islets. In: Joost, HG., Al-Hasani, H., Schürmann, A. (eds) Animal Models in Diabetes Research. Methods in Molecular Biology, vol 933. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-068-7_12
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DOI: https://doi.org/10.1007/978-1-62703-068-7_12
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