Skip to main content
SpringerLink
Log in

Effect of hormones and cyclic AMP on γ-glutamyltranspeptidase activity of rat mammary gland explants

  • Short Communications
  • Biochemistry, Biophysics, Metabolism
  • Published:
Experientia Aims and scope Submit manuscript

Summary

γ-Glutamyl transpeptidase activity was assayed in midpregnant rat mammary gland explants at 14, 22 and 38 h, in the presence and absence of insulin, prolactin and corticosterone. With these 3 hormones the explants attained the characteristics of a secretory gland after 22 h of tissue culture, at which time the enzyme exhibited its maximal activity. The addition of dibutyryl cyclic AMP in the presence of the 3 hormones produced a significant increase in enzyme activity, which was maximal with a 1 mM concentration of the cyclic nucleotide. A similar effect was observed when theophylline or theophylline plus dibutyryl cyclic AMP were added to the culture medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Meister, A., Tate, S.S., and Ross, L.L., in: The enzymes of Biological Membranes, p. 315. Ed. A. Martinosi. Plenum Press, New York 1976.

    Chapter  Google Scholar 

  2. Wood, J.L., in: Metabolic Conjugation and Metabolic Hydrolysis, vol. 2, p. 261. Ed. W.H. Fishman. Academic Press, New York 1970.

    Chapter  Google Scholar 

  3. Meister, A., and Tate, S.S., A. Rev. Biochem.45 (1976) 559.

    Article  CAS  Google Scholar 

  4. Puente, J., Varas, M.A., Beckhaus, G., and Sapag-Hagar, M., FEBS Lett.99 (1979) 215.

    Article  CAS  PubMed  Google Scholar 

  5. Puente, J., Castellón, E., and Sapag-Hagar, M., Experientia38 (1982) 531.

    Article  CAS  PubMed  Google Scholar 

  6. Puente, J., Castellón, E., and Sapag-Hagar, M., Biochem. J.207 (1982) 605.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Topper, Y.J., Oka, T., and Vonderhaar, B.K. in: Methods in Enzymology, vol. 39, p. 443. Ed. J.G. Hardman and B.W. O'Malley. Academic Press, New York 1975.

    Google Scholar 

  8. Tate, S.S., and Meister, A., J. biol. Chem.249 (1974) 7592.

    Article  Google Scholar 

  9. Kurz, G., and Wallenfels, K., in: Methods of Enzymatic Analysis, vol. 3, p. 1180. Ed. H.U. Bergmeyer. Verlag Chemie, Weinheim/New York 1974.

    Google Scholar 

  10. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.S., J. biol. Chem.193 (1951) 265.

    Article  CAS  PubMed  Google Scholar 

  11. Stockdale, F.E., and Topper, Y.J., Proc. natl Acad. Sci. USA56 (1966) 1283.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Sapag-Hagar, M., Greenbaum, A.L., Lewis, D.J., and Hallowes, R.C., Biochem. biophys. Res. Commun.59 (1974) 261.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Bioquímica, Facultad de Ciencias Básicas y Farmacéuticas, Universidad de Chile, Casilla 233, Santiago 1, Chile

    M. P. Valdivieso, J. F. Puente & M. Sapag-Hagar

Authors
  1. M. P. Valdivieso
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. F. Puente
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Sapag-Hagar
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by grant B1138-8333 from the Departamento de Desarrollo de la Investigación, Universidad de Chile.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Valdivieso, M.P., Puente, J.F. & Sapag-Hagar, M. Effect of hormones and cyclic AMP on γ-glutamyltranspeptidase activity of rat mammary gland explants. Experientia 40, 710–711 (1984). https://doi.org/10.1007/BF01949736

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01949736

Keywords

Search

Navigation