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Selection for resistance toBacillus thuringiensis δ-endotoxin in an insect cell line (Choristoneura fumiferana)

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Summary

A cell line of the spruce budworm (FPMI-CF1) consists of a mixed population of cells that possess variable sensitivity to δ-endotoxin from crystals ofBacillus thuringiensis. A cell strain was selected from FPMI-CF1 which was resistant to the entomocidal protein extracted fromB. thuringiensis crystals. The resistant character was unstable, however, and could not be maintained in the absence of toxin during growth.

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Authors and Affiliations

  1. U.S. Grain Marketing Research Laboratory, Agricultural Research Service, USDA, 66502, Manhattan, Kansas, USA

    D. E. Johnson

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  1. D. E. Johnson
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Additional information

The author wishes to thank S.S. Sohi (Forest Pest Management Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario, Canada) for theC.fumiferana cell line, and L.I. Davidson (USDA) for his able technical assistance.

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Johnson, D.E. Selection for resistance toBacillus thuringiensis δ-endotoxin in an insect cell line (Choristoneura fumiferana). Experientia 40, 274–275 (1984). https://doi.org/10.1007/BF01947579

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  • DOI: https://doi.org/10.1007/BF01947579

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