Summary
Lysine content in bread wheat (Triticum aestivum L. m. Thell) was determined by a modified Udy-dye binding method and was compared with that obtained from the amino acid analyzer. The values obtained from the 2 methods were correlated and the co-efficient was found to be +0.91 at 0.450 mg/ml dye concentration. The modified method is quick, less expensive and quite helpful for screening lysine at earlier generation in wheat breeding for improved grain quality content.
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References
M.J. Lewrence, J.M. Day, M. Katherine, H. Edith and Barbara Lee, Cereal Chem.35, 169 (1958).
E.E. Dermot and J. Pace, J. Sci. Fd Agric.11, 109 (1960).
E. Villegas, C.E. Mc Donald and K.A. Giles, Am. Ass. Cereal Chem.47, 147 (1970).
Y. Pomeranz and B.S. Miller, J. off. agric. Chem45, 399 (1963).
M.L. Kakade and I.E. Liner, Analyt. Biochem.27, 273 (1969).
R. Mossberg, in: New approaches to breeding for improved plant protein, p. 151. Int. Atomic Energy Agency, Vienna 1969.
F.P. Zscheila, Jr, and B.L. Braman, Analyt. Biochem.49, 442 (1972).
R.S. Bhatty and K.K. Wu, Can. J. Pl. Sci.55, 685 (1975).
D.C. Udy, Cereal Chem.33, 190 (1956).
D.C. Udy, J. Am. Oil Chem. Soc.48, 29 (1971).
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The author is grateful to Dr V.A. Johnson, University of Nebraska, Lincoln, USA, for supplying wheat cultivars with known protein and lysine values.
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Khan, M.I. Lysine estimation with the modified Udy-dye binding method in hexaploid wheat. Experientia 34, 711–712 (1978). https://doi.org/10.1007/BF01947274
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DOI: https://doi.org/10.1007/BF01947274