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Oxidative phosphorylation inTrypanosoma cruzi

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Zusammenfassung

In Epimastigoten vonT. cruzi wird die oxydative Phosphorylierung bewiesen durch: 1. Beseitigung der Atmungskontrolle durch Zugabe von entkoppelnden Substanzen (CCP und PCP); 2. Hemmung der Phosphorylierung durch Antimycin mit gleichzeitiger Reduktion von Cytochrom ‘b’; 3. Atmungshemmung durch Dicyclohexylcarbodiimid (DCCD); 4. Verminderung des intrazellulären ATP durch CCP, DCCD und Cyanid.

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Abbreviations

PCP:

pentachlorophenol

CCP:

carbonylcyanide-m-chlorophenyl hydrazone

DCCD:

N, N′-dicyclohexylcarbodiimide

Pi :

inorganic orthophosphate

P7 :

heat and acid labile phosphate

Pas :

total acid-soluble phosphate

Po :

Pas less (Pi+P7)

References

  1. G. C. Hill andW. A. Anderson, Expl Parasit.28, 356 (1970).

    Article  CAS  Google Scholar 

  2. G. C. Hill, inComparative Biochemistry of Parasites (Ed.H. van den Bossche; Academic Press, New York and London 1972), p. 395.

    Chapter  Google Scholar 

  3. J. P. Kusel andB. Storey, Biochem. biophys. Res. Commun.46, 501 (1972).

    Article  CAS  Google Scholar 

  4. J. J. Toner andM. M. Weber, Biochem. biophys. Res. Commun.46, 652 (1972).

    Article  CAS  Google Scholar 

  5. S. K. Ray andG. A. M. Cross, Nature, New Biol.237, 174 (1972).

    Article  CAS  Google Scholar 

  6. J. F. de Boiso andA. O. M. Stoppani, Proc. Soc. exp. Biol. Med.136, 215 (1971).

    Article  Google Scholar 

  7. J. F. de Boiso andA. O. M. Stoppani, Experientia28, 1162 (1972).

    Article  Google Scholar 

  8. T. von Brand, Revta. Inst. Med. trop. S. Paulo4, 53 (1962).

    Google Scholar 

  9. H. Adam, inMethods of Enzymatic Analysis (Ed.H.-U. Bergmayer; Academic Press, New York and London 1963), p. 539.

    Google Scholar 

  10. J. F. Ryley, Biochem. J.62, 215 (1956).

    Article  CAS  Google Scholar 

  11. J. D. Fulton andD. F. Spooner, Exp. Parasit.8, 137 (1959).

    Article  CAS  Google Scholar 

  12. B. Chance, Fedn. Europ. biochem. Soc. Lett.23, 3 (1972).

    Article  CAS  Google Scholar 

  13. J. R. Brandon, J. R. Brockelhurst andC. P. Lee, Biochemistry11, 1150 (1972).

    Article  CAS  Google Scholar 

  14. L. Kovac, T. Galeotti andB. Hess, Biochim. biophys. Acta153, 715 (1968).

    Article  CAS  Google Scholar 

  15. C. Long,Biochemists' Handbook (E. and F. N. Spon Ltd., London 1961), p. 783.

    Google Scholar 

  16. L. F. Leloir andC. E. Cardini, inMethods in Enzymology (Eds.S. P. Colowick andN. O. Kaplan; Academic Press, New York 1957), vol. 3, p. 840.

    Chapter  Google Scholar 

  17. M. B. R. de Sastre andA. O. M. Stoppani, Fedn. Europ. biochem. Soc. Lett.31, 137 (1973).

    Article  Google Scholar 

  18. A. Sanabria, expl Parasit.19, 276 (1966).

    Article  CAS  Google Scholar 

  19. K. Shibata, A. A. Benson andM. Calvin, Biochem. biophys. Acta15, 461 (1954).

    Article  CAS  Google Scholar 

  20. This investigation was supported by grants from Consejo Nacional de Investigaciones Cientificas y Técnicas (Argentina), Instituto Nacional de Farmacologia and the Wellcome Trust. We are grateful to ProfessorE.D.P. de Robertis for allowing the use of the splitbeam spectrophotometer and toM.B.R. de Sastre for his assistance with the measurements of ATP.

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Authors and Affiliations

  1. Instituto de Química Biológica, Facultad de Medicina, Paraguay 2155, Buenos Aires, Argentina

    A. O. M. Stoppani & J. F. de Boiso

Authors
  1. A. O. M. Stoppani
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  2. J. F. de Boiso
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Career Investigator, CONICET.

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Stoppani, A.O.M., de Boiso, J.F. Oxidative phosphorylation inTrypanosoma cruzi. Experientia 29, 1494–1496 (1973). https://doi.org/10.1007/BF01943876

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