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Single channel recording and gating function of ionic channels

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Summary

This review discusses several aspects of the kinetic analysis of the gating function of membrane channels, as carried out on single channel data obtained by the patch clamp method. In particular, the following three aspects of channel behavior are reviewed in some detail: 1) estimate of the number of states or conformations the channel can enter; 2) the lifetime of each state as a function of transmembrane voltage (voltage-gated channels), or of ligand concentration (ligand-gated channels); 3) estimates of the rates at which the transitions between the various states occur, and assessment of reaction mechanisms. An introductory description of conformational transitions of channel proteins is also provided.

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References

  1. Armstrong, C. M., and Bezanilla, F., Currents related to movement of the gaging particles of the sodium channels. Nature242 (1973) 459–461.

    Article  CAS  PubMed  Google Scholar 

  2. Barrett, J. N., Magleby, K. L., and Pallotta, B. S., Properties of single calcium-activated potassium channels in cultured rat muscle. J. Physiol.331 (1982) 211–230.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Blatz, A. L., and Magleby, K. L., Correcting single channel data for missed events. Biophys. J.49 (1986) 967–980.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Colquhoun, D., and Hawkes, A. G., On the stochastic properties of single ion channel. Proc. R. Soc.211 (1981) 205–235.

    CAS  Google Scholar 

  5. Colquhoun, D., and Sigworth, F. J., Fitting and statistical analysis of single-channel records, in: Single-Channel Recording, pp. 191–263. Eds B. Sakmann, and E. Neher. Plenum Press, New York 1983.

    Chapter  Google Scholar 

  6. Conti, F., and Wanke, E., Channel noise in nerve membranes and lipid bilayers. Q. Rev. Biophys.8 (1975) 451–505.

    Article  CAS  PubMed  Google Scholar 

  7. Dahiya, R. C., An improved method of estimating an integer parameter by maximum likelihood. Am. Statistician35 (1981) 34–47.

    Google Scholar 

  8. Eyring, H., The activated complex in chemical reactions. J. Chem. Phys.3 (1935) 107–115.

    Article  CAS  Google Scholar 

  9. Franciolini, F., Patch clamp technique and biophysical study of membrane channels. Experientia42 (1986) 589–594.

    Article  CAS  PubMed  Google Scholar 

  10. Glasstone, S., Laidler, K. J., and Eyring, H., The Theory of Rate Processes, pp. 611. McGraw-Hill, New York 1941.

    Google Scholar 

  11. Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J., Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch.391 (1981) 85–100.

    Article  CAS  PubMed  Google Scholar 

  12. Hille, B., Ionic Channels of Excitable Membranes, p. 426. Sinauer Associates Inc., Sunderland, Mass. 1984.

    Google Scholar 

  13. Hodgkin, A. L., and Huxley, A. F., Currents curried by sodium and potassium ions through the membrane of the giant axon ofLoligo. J. Physiol.116 (1952) 473–496.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Horn, R., and Lange, K., Estimating kinetic constants from single channel data. Biophys. J.43 (1983) 207–223.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Karplus, M., Dynamics of proteins. Ber. Bunsenges. Phys. Chem.86 (1982) 386–895.

    Article  CAS  Google Scholar 

  16. Keynes, R. D., and Rojas, E., Kinetics and steady-state properties of the charged system controlling sodium conductance in the squid giant axon. J. Physiol.239 (1974) 393–434.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Magleby, K. L., and Pallotta, B. S., Dependence of open and shut interval distributions from calcium-activated potassium channels in cultured rat muscle. J. Physiol.344 (1983) 585–604.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Moore, J. W., and Pearson, R. G., Kinetics and Mechanisms, 3rd edn, pp. 455. John Wiley, New York 1981.

    Google Scholar 

  19. Neher, E., and Sakmann, B., Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature260 (1976) 799–802.

    Article  CAS  PubMed  Google Scholar 

  20. Neher, E., and Stevens, C. F., Conductance fluctuations and ionic pores in membranes. A. Rev. Biophys. Bioeng.6 (1977) 345–381.

    Article  CAS  Google Scholar 

  21. Patlak, J., The information content of single channel data, in: Membranes, Channels, and Noise, pp. 179–234. Eds R. S. Eisenberg, M. Frank and C. F. Stevens. Plenum Press, New York 1984.

    Google Scholar 

  22. Roux, B., and Sauve', R., A general solution to the time interval omission problem applied to single channel analysis. Biophys. J.48 (1985) 149–158.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Sachs, F., Neil, J., and Bakakati, N., The automated analysis of data from single ionic channels. Pflügers Arch.395 (1982) 331–340.

    Article  CAS  PubMed  Google Scholar 

  24. Schneider, M. F., and Chandler, W. K., Voltage-dependent charge movement in skeletal muscle: A possible step in excitation-contraction coupling. Nature242 (1973) 244–246.

    Article  CAS  PubMed  Google Scholar 

  25. Sigworth, F. J., The patch clamp is more useful than anyone had expected. Fedn Proc.45 (1986) 2673–2677.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Miami School of Medicine, POB 016430, 33101, Miami, Florida, USA

    F. Franciolini

  2. Istituto Biologia Cellulare Università Perugia, Via Elce di Sotto 1, I-06100, Perugia, Italy

    A. Petris

Authors
  1. F. Franciolini
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  2. A. Petris
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Franciolini, F., Petris, A. Single channel recording and gating function of ionic channels. Experientia 44, 183–188 (1988). https://doi.org/10.1007/BF01941702

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  • DOI: https://doi.org/10.1007/BF01941702

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