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Isolation and characterization of a new hemagglutinin from the red algaGracilaria bursa-pastoris

Experientia volume 46, pages 975–977 (1990)Cite this article

Summary

A new agglutinin has been isolated from the red algaGracilaria bursa-pastoris by affinity chromatography on a yeast mannan-Cellulofine column. This agglutinin was isolated as a monomeric glycoprotein with a relatively low molecular weight. It had an isoelectric point of 4.7 and contained large amounts of Gly, Asx and Glx. It agglutinated trypsin-treated rabbit erythrocytes at the low concentration of 30 ng/ml. The activity was inhibited only by glycoproteins bearing N-glycans. This agglutinin also showed mitogenic activity for mouse splenic lymphocytes.

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Authors and Affiliations

  1. Faculty of Applied Biological Science, Hiroshima University, 724, Higashi-Hiroshima, (Japan)

    R. Okamoto, K. Hori, K. Miyazawa & K. Ito

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  1. R. Okamoto
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  2. K. Hori
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  3. K. Miyazawa
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  4. K. Ito
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Okamoto, R., Hori, K., Miyazawa, K. et al. Isolation and characterization of a new hemagglutinin from the red algaGracilaria bursa-pastoris . Experientia 46, 975–977 (1990). https://doi.org/10.1007/BF01939393

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  • DOI: https://doi.org/10.1007/BF01939393

Key words

  • Marine alga
  • agglutinin
  • mitogen
  • glycoprotein
  • sugar-binding specificity
  • biological activity