Summary
To explain the mechanism of DNP action the contents of ATP, ADP, AMP and G-6-P were determined in perfused rat hearts in a period of time in which the rate of the glycogenolysis was increased. The levels of these metabolytes in the extract were consistent with an increase of the phosphofructokinase activity. On the other hand, the finding of higher activity of phosphorylaseb kinase in DNP-poisoned animals could be explained as due to the low content of G-6-P in the perfused hearts subjected to the action of the drug.
Similar content being viewed by others
Literatur
A. Focesi Jr., Q. S. Tahin, M. M. El-Guindy andA. E. Vercesi, Experientia25, 1243 (1969).
A. E. Vercesi andA. Focesi jr., Experientia29, 392 (1973).
A. E. Vercesi, Doctorate Thesis (1974).
E. Helmreich, S. Karpatkim andC. F. Cori, in Ciba Foundation Symposium (1964), p. 211.
W. F. Loomis andF. Lipmann, J. biol. Chem.173, 807 (1948).
H. E. Morgan, M. J. Henderson, D. M. Regen andC. R. Park, J. biol. Chem.236, 253 (1961).
A. M. Petersen andH. M. Kalckar, inMethods in Enzymology (Eds.S. P. Colowick andN. O. Kaplan; Academic Press Inc., New York 1957), vol. 3, p. 869.
G. Nikiforouk andP. Colowick, inMethods in Enzymology (Eds.S. P. Colowick andN. O. Kaplan; Academic Press Inc., New York 1955), vol. 2, p. 469.
L. Noda andS. A. Kuby, inMethods in Enzymology (Eds.S. P. Colowick andN. O. Kaplan; Academic Press Inc., New York 1963), vol. 6, p. 223.
P. S. Krishman, inMethods in Enzymology (Eds.S. P. Colowick andN. O. Kaplan; Academic Press Inc., New York 1955), vol. 2, p. 591.
B. L. Horecker andW. A. Wood, inMethods in Enzymology (Eds.S. P. Colowick andN. O. Kaplan; Academic Press Inc., New York 1957), vol. 3, p. 152.
E. G. Krebs, D. S. Love, G. E. Bratvold, K. A. Trayser, W. L. Meyer andE. H. Fischer, Biochemistry3, 1022 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vercesi, A.E., Focesi, A. On the mechanism of the glycogenolytic effect of dinitrophenol. Activity of phosphofructokinase in perfused hearts. Experientia 31, 771–772 (1975). https://doi.org/10.1007/BF01938454
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01938454