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Esterases of the flounder (Platichthys flesus, Pleuronectidae, Teleostei): Development of an identification protocol using starch gel electrophoresis and characterization of loci

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Summary

Fish esterases are among the most difficult enzymes to identify using starch gel electrophoresis because of the many loci that are simultaneously active, and especially because of duplication phenomena, satellite bands, and stain trails. In an attempt to simplify and clarify electropherograms, various staining and inhibitory methods were tested on esterases from the flounderPlatichthys flesus. A range of migration and staining buffers and substrates were used, as well as chemical inhibition and heat inactivation. A combination of the methods made it possible to distinguish and characterize the five presumed esterase loci.

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Authors and Affiliations

  1. Laboratoire de Génétique de l'Institut des Sciences de l'Evolution (URA 327, CNRS), Université Montpellier II, Place E. Bataillon, F-34095, Montpellier Cedex 5, France

    P. Berrebi, P. Landaud, P. Borsa & J. F. Renno

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  1. P. Berrebi
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  2. P. Landaud
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  3. P. Borsa
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  4. J. F. Renno
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Berrebi, P., Landaud, P., Borsa, P. et al. Esterases of the flounder (Platichthys flesus, Pleuronectidae, Teleostei): Development of an identification protocol using starch gel electrophoresis and characterization of loci. Experientia 46, 863–867 (1990). https://doi.org/10.1007/BF01935540

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  • DOI: https://doi.org/10.1007/BF01935540

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