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Über das Vorkommen von Carboanhydratase in der Eischale des Huhnes

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Summary

The isolation of carbonic anhydrase from hen's egg shells is reported. The enzyme has been purified by gelfiltration and ion-exchange chromatography. The elution pattern of carbonic anhydrase from egg shells differs remarkably from that of enzyme preparations of erythrocytes. The purified enzyme catalyzed the reversible hydration of CO2 and aldehydes as well as the hydrolysis ofp-nitrophenyl acetate. Deletion of 2-mercaptoethanol from extraction and elution buffer solutions caused a complete loss of carbonic anhydrase activity, as did the addition ofp-mercuribenzoate. The biological significance of the occurance of carbonic anhydrase in the hen's egg shell is discussed.

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Authors and Affiliations

  1. Forschungs- und Lehrbereich Biochemie, Institut für Anatomie, Physiologie und Hygiene der Haustiere, Universität Bonn, Katzenburgweg 7, D-53, Bonn, Deutschland

    G. Krampitz, J. Engels & I. Helfgen

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  1. G. Krampitz
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  2. J. Engels
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  3. I. Helfgen
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Wir danken der Deutschen Forschungsgemeinschaft für die Förde rung dieser Untersuchungen.

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Krampitz, G., Engels, J. & Helfgen, I. Über das Vorkommen von Carboanhydratase in der Eischale des Huhnes. Experientia 30, 228–229 (1974). https://doi.org/10.1007/BF01934795

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  • DOI: https://doi.org/10.1007/BF01934795

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