Abstract
The hemolymph from various species of moths was analyzed for cross-reactivity with a panel of six monoclonal antibodies made against the hemolymph juvenile hormone binding protein ofManduca sexta. With the exception of one antibody, the immunoreactivity was limited to the sphingid family. One monoclonal antibody cross-reacted with a number of lepidopteran species; however, families such as Noctuidae and Pyralidae, known to have high affinity, low molecular weight juvenile hormone binding proteins, did not cross-react. Immunological cross-reactivity withManduca sexta juvenile hormone binding protein in several primitive moth families supports the current model of phylogenetic relationships in the order Lepidoptera.
Similar content being viewed by others
References
Goodman, W. G., in: Morphogenetic Hormones of Arthropods, pp. 83–124. Ed. A. P. Gupta. Rutgers University Press, New Brunswick 1990.
Goodman, W. G., and Chang, E. S., in: Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol. 7, pp. 491–510. Eds G. A. Kerkut and L. I. Gilbert. Pergamon Press, Oxford 1985.
Koeppe, J. K., and Kovalick, G. E., in: Biochemical Actions of Hormones, vol. 13, pp. 266–304. Ed. G. Litwack. Academic Perss, Orlando 1986.
Hodges, R. W., Checklist of the Lepidoptera of America North of Mexico. E. W. Classey Ltd, London 1983.
Lammeli, U. K., Nature227 (1970) 680.
Hames, B. D., in: Introduction to Polyacrylamide Gel Electrophoresis, pp. 1–91. Eds B. D. Hames and D. Rickwood. IRL Press, Oxford 1981.
Goodman, W. G., Park, Y. C., and Johnson, J., Insect Biochem.20 (1990) 611.
Hancock, K., and Tsang, V. C. W., Analyt. Biochem.133 (1983) 157.
Schooley, D. A., Baker, F. C., Tsai, L. W., Miller, C. A., and Jamieson, G. C., in: Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones, pp. 373–383. Eds J. Hoffmann and M. Porchet. Springer-Verlag, Berlin 1984.
Ryan, R. O., Smidt, J. O., and Law, J. H., Arch. Insect Biochem.1 (1984) 375.
Laver, W. G., Gillian, M. A., Webster, R. G., and Smith-Gill, S. J., Cell61 (1990) 553.
Brock, J. P., J. nat. Hist.5 (1971) 29.
Common, I. F. B., A. Rev. Ent.20 (1975) 183.
Prestwich, G. D., Science (Wash. D. C.)237 (1987) 999.
Covell, C. V., A Field Guide to the Moths of Eastern North America. Houghton Mifflin, Boston 1984.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Goodman, W.G., Maxfield, B. & Park, Y.C. Immunochemical similarities among the hemolymph juvenile hormone binding proteins of moths. Experientia 47, 945–948 (1991). https://doi.org/10.1007/BF01929888
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01929888