Summary
2 prothoracotropical factors (activation factor I and II) have been obtained by gel filtration techniques from brains and corpora cardiaca of the cockroachPeriplaneta americana. In contrast to activation factor II, activation factor I caused significant influence of RNA synthesis. The RNA pattern of prothoracic glands stimulated by activation factor I as demonstrated by polyacrylamide gel electrophoresis consists of different kinds of RNA. Short time incubation revealed effects on sRNA synthesis, while long time incubation demonstrated predominantly increase of ribosomal RNA synthesis. Measurements of the membrane potential of the prothoracic gland cells of the wax mothGalleria mellonella indicated an increase by activation factor II; activation factor I was without any visible effect. Our results demonstrate for the first time that the two activation factors induce different effects at cellular level.
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Für technische Unterstützung danken wir FräuleinA. Zinsser und FrauR. Meissner.
Durchgeführt mit Mitteln des Ministeriums für Wissenschaft und Technik und mit Unterstützung durch die Sächsische Akademie der Wissenschaften zu Leipzig.
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Gersch, M., Bräuer, R. & Birkenbeil, H. Experimentelle Untersuchungen zum Wirkungsmechanismus der beiden entwicklungsphysiologisch aktiven Fraktionen des «Gehirnhormons» der Insekten (Aktivationsfaktor I und II) auf die Prothoracaldrüse. Experientia 29, 425–427 (1973). https://doi.org/10.1007/BF01926759
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DOI: https://doi.org/10.1007/BF01926759