Abstract
Although dipyridamole has been extensively studied as an anti-aggregating agent, its mechanism of action has not been elucidated. Cultured mesangial cells were treated with dipyridamole 1–100 μM from 6–72 h. Ecto-5′-nucleotidase activity approximately doubled (from 115±11 to 226±14 nmol/min/mg) after treatment with 100 μM dipyridamole for 72 h. This effect was concentration- and time-dependent. Cycloheximide, an inhibitor of protein synthesis, did not alter basal 5′-nucleotidase activity. However, it prevented stimulation by 5 μM dipyridamole. Adenosine availability at the receptor sites was increased by dipyridamole and S-(p-nitrobenzyl)-6-thioinosine (NBTI), which inhibit adenosine uptake into the cell. Addition of dipyridamole or NBTI to the adenosine-treated mesagial cells produced an additive increase in ecto-5′-nucleotidase activity. Dipyridamole, through its effect on extracellular adenosine and ecto-5′-nucleotidase, may have an influence upon regulation of the glomerular microcirculation.
Similar content being viewed by others
References
Born, G. V. R., and Mills, D. C. B., J. Physiol., Lond.202 (1969) 41.
Mills, D. C. B., and Smith, J. B., Biochem. J.121 (1971) 185.
Moncada, S., and Korbut, R., LancetI (1978) 1286.
Berne, R. M., Circulation Res.47 (1980) 807.
Stefanović, V., Savić, V., Vlahović, P., Ardaillou, N., and Ardaillou, R., Renal Physiol. Biochem.11 (1988) 89.
Baud, L., Hagege, J., Sraer, J., Rondeau, E., Perez, J., and Ardaillou, R., J. expl Med.158 (1983) 1836.
Gomori, G., J. Lab. clin. Med.27, (1942) 955.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R., J. biol. Chem.193 (1951) 265.
Stefanović, V., Vlahović, P., Savić, V., Ardaillou, N., and Ardaillou, R., FEBS Lett.331 (1993) 96.
Savić, V., Blanchard, A., Vlahović, P., Stefanović, V., Ardaillou, N., and Ardaillou, R., Archs Biochem. Biophys.290 (1991) 202.
Olivera, A., Lamas, P., Rodrigues-Puyol, D. and Lopez-Novoa, J. M., Kidney Intl35 (1989) 1300.
Osswald, H., Spielman, W. S., and Knox, F. G., Circulation Res.43 (1978) 465.
Skott, O., and Baumbach, L., Pflugers Arch.404 (1985) 232.
Kincaid-Smith, P., Am. J. Kidney Dis.3 (1984) 299.
Cameron, J. S., Kidney Intl44 suppl. 42 (1993) S2.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stefanović, V., Savić, V. & Vlahović, P. Effect of dipyridamole on glomerular mesangial cell ecto-5′-nucleotidase expression. Experientia 50, 943–946 (1994). https://doi.org/10.1007/BF01923484
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01923484