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The C4-dicarboxylate transport system ofRhizobium meliloti and its role in nitrogen fixation during symbiosis with alfalfa (Medicago sativa)

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  • Symbiotic Interactions Between Microorganisms and Plants
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Abstract

TheRhizobium meliloti C4-dicarboxylate transport (Dct) system is essential for an effective symbiosis with alfalfa plants. C4-dicarboxylates are the major carbon source taken up by bacteroids. Genetic analysis of Dct− mutant strains led to the isolation of thedct carrier genedctA and the regulatory genesdctB anddctD. The carrier genedctA is regulated in free-living cells by the alternative sigma factor RpoN and the two-component regulatory system DctB/D. In addition, DctA is involved in its own regulation, possibly by interacting with DctB. In bacteroids, besides the DctB/DctD system an additional symbiotic activator is thought to be involved indctA expression. Further regulation ofdctA in the free-living state is reflected by diauxic growth of rhizobia, with succinate being the preferred carbon source. The tight coupling of C4-dicarboxylate transport and nitrogen fixation is revealed by a reduced level of C4-dicarboxylate transport in nitrogenase negative bacteroids.

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  1. Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Postfach 10 01 31, D-33501, Bielefeld, Germany

    D. Jording, C. Uhde, R. Schmidt & A. Pühler

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Jording, D., Uhde, C., Schmidt, R. et al. The C4-dicarboxylate transport system ofRhizobium meliloti and its role in nitrogen fixation during symbiosis with alfalfa (Medicago sativa). Experientia 50, 874–883 (1994). https://doi.org/10.1007/BF01923473

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