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Isolation and properties of cell walls fromAgrobacterium tumefaciens B 6 1

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Summary

Purified cell walls were prepared fromAgrobacterium tumefaciens B 6 by extraction of intact cells with hot sodium dodecyl sulfate and digestion with proteases. Such preparations contained peptidoglycan that accounted for about 40% of their dry weight. Electron micrographs of the purified walls showed that they conserved their characteristic shape despite the drastic extraction procedure.

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Authors and Affiliations

  1. Department of Biophysics, The Weizmann Institute of Science, Rehovot, (Israel)

    D. Levanon

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  1. D. Levanon
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Additional information

This work was supported in part by a contribution from a friend of the Weizmann Institute of Science in Buenos Aires, Argentine.

I wish to thank Professor Nathan Sharon for his constant interest and critical discussion during this work.

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Levanon, D. Isolation and properties of cell walls fromAgrobacterium tumefaciens B 6 1 . Experientia 34, 354–356 (1978). https://doi.org/10.1007/BF01923031

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  • DOI: https://doi.org/10.1007/BF01923031

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