Summary
The effects of β-adrenergic stimulation produced by an infusion of isoproterenol (1 μg·kg−1 min−1, 30 min) were studiedin situ in the anaesthetized dog placed under a total cardiopulmonary bypass. Samples of the subepicardial and the subendocardial layers were homogenized separately prior to the extraction and methylation of free fatty acids (FFA). Gas chromatography on Carbowax 20 M capillary columns was used for the quantitation of myristic (C 14∶0), palmitic (C 16∶0), palmitoleic (C 16∶1), stearic (C 18∶0), oleic (C 18∶1), linoleic (C 18∶2), and arachidonic (C 20∶4) acids.
Within 5 min, isoproterenol decreased the tissue content of FFA significantly. The decrease was more pronounced in the endocardial layer where the FFA concentration reached its minimum at the 5th or the 15th min. In the epicardial layer, all the FFA reached their minimal concentration at the 30th min of the isoproterenol infusion. In both layers, lactate content remained unchanged at 5 and 15 min and rose at the 30th min only and content in phosphorylated compounds (ATP, creatine-phosphate—CP) did not show any significant variation during the β-stimulation period. A significant correlation was found between the chronotropic effect of isoproterenol and the reduction of FFA concentration.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Al Makdessi S, Andrieu JL, Timour Chah Q, Tuduri AJ, Faucon G (1982) Relationship between lactate arteriovenous gradient and tissue lactate content in subendocardial and subepicardial layers of the ischaemicin situ heart. Cardiovasc Res 16:552–558
Andrieu JL, Vial C, Font B, Goldschmidt D, Ollagnier M, Faucon G (1980) Effects of isoproterenol on the metabolism of normal and ischemic heart. Arch Int Pharmacodyn Ther 244:255–269
Bergmeyer HU (1965) Methods of enzymatic analysis. Academic Press, New York, p 260–270
Bergmeyer HU (1974) Methods of enzymatic analysis. Academic Press, New York, p 1777–1781.
Borensztajn J, Robinson DS (1970) The effect of fasting on the utilization of chylomicron triglyceride fatty acids in relation to clearing factor lipase (lipoprotein lipase) releasable by heparin in the perfused rat heart. J Lip Res 11:111–117
Crass III MF, McCaskill ES, Shipp JC, Murthy VK (1971) Metabolism of endogenous lipids in cardiac muscle: effect of pressure development. Am J Physiol 220:428–435
Crass III MF, Shipp JC, Pieper GM (1975) Effects of catecholamines on myocardial endogenous substrates and contractility. Am J Physiol 228:618–627
Crass III MF (1977) Regulation of triglyceride metabolism in the isotopically prelabelled perfused heart. Fed Proc 36:1995–1999
Duncombe WG (1964) The colorimetric micro determination of non esterified fatty acids in plasma. Clin Chim Acta 9:122–125
Griggs DM (1979) Blood flow and metabolism in different layers of the left ventricle. The Physiologist 22:36–40
Hochachka PW, Neely JR, Driedzic WR (1977) Integration of lipid utilization with Krebs cycle activity in muscle. Fed Proc 36:2009–2014
Höckel M, Dünges W, Holzer A, Brockerhoff P, Rathgen GM (1980) A microliter method for the gas chromatographic determination of long-chain nonesterified fatty acids in human serum or plasma. J Chromatogr 221:205–214
Hunneman DH, Schweickhardt C (1982) Mass fragmentographic determination of myocardial free fatty acids. J Mol Cell Cardiol 14:339–351
Kjekshus J, Mjøs OD (1972) Effect of increased afterload on the inotropic state and uptake of free fatty acids in the intact dog heart. Acta Physiol Scand 84:415–427
Kreisberg RA (1966) Effect of epinephrine on myocardial triglyceride and free fatty acid utilization. Am J Physiol 210:385–389
Lech JJ, Jesmok GJ, Calvert DN (1977) Effects of drugs and hormones on lipolysis in heart. Fed Proc 36:2000–2008
Masters TN, Glaviano VV (1972) The effects of norepinephrine and propranolol on myocardial subcellular distribution of triglycerides and free fatty acids. J Pharmacol Exp Ther 182:246–255
Mjøs OD (1971) Effect of inhibition of lipolysis on myocardial oxygen consumption in the presence of isoproterenol. J Clin Invest 50:1869–1873
Mueller HW, Binz K (1982) Glass capillary gas chromatography of the serum fatty acid fraction via automatic injection of lipid extracts. J Chromatogr 228:75–93
Opie LH, Owen P, Thomas M, Samson R (1973) Coronary sinus lactate measurements in assessment of myocardial ischemia. Am J Cardiol 32:295–305
Oram JF, Bennetch SL, Neely JR (1973) Regulation of fatty acid utilization in isolated perfused rat hearts. J Biol Chem 248:5299–5309
Robinson DS, Jennings MA (1965) Release of clearing factor lipase by the perfused rat heart. J Lip Res 6:222–227
Sampson D, Hensley WJ (1975) A rapid gas chromatographic method for the quantitation of underivatised individual free fatty acids in plasma. Clin Chim Acta 61:1–8
Schwartz K, Rey P, Bui-Mong-Hung, De Mendonca M (1973) A simple drill biopsy technique: evaluation for muscular and myocardial biochemical measurements. J Mol Cell Cardiol 5:235–246
Severson DL (1979) Regulation of lipid metabolism in adipose tissue and heart. Can J Physiol Pharmacol 57:923–937
Van der Vusse GJ, Roemen TH, Prinzen FW, Coumans WA, Reneman RS (1982) Uptake and tissue content of fatty acids in dog myocardium under normoxic and ischemic conditions. Circ Res 50:538–546
Vik-Mo H, Mjøs OD (1981) Influence of free fatty acids on myocardial oxygen consumption and ischemic injury. Am J Cardiol 48:361–365
Author information
Authors and Affiliations
Additional information
With the technical assistance of Agnès Bacconin.
Rights and permissions
About this article
Cite this article
AlMakdessi, S., Herilier, H., Andrieu, J.L. et al. Effect of β-adrenergic stimulation on free fatty acid content in subepicardial and subendocardial layers of the dog heart in situ. Separation and assay by capillary gas chromatography. Basic Res Cardiol 80, 182–190 (1985). https://doi.org/10.1007/BF01910466
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01910466