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Uncoupler-and hypoxia-induced damage in the working rat heart and its treatment

I. Observations with uncouplers of oxidative phosphorylation

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Summary

In the isolated working rat heart, the damaging effect of 0.05–0.06 μM Carbonylcyanide-p-trifluoro-methoxyphenylhydrazone (FCCP) was reversible within 20 sec by perfusion with fresh buffer. Cysteine 3 mM restored the aortic flow to the initial value within an additional 15–20 sec. Thereafter, the FCCP effect became irreversible due to a progressive structural membrane change. The structural change by FCCP is probably brought about by ‘internalization’ of polar groups (R-SH; R−NH3 +) of the mitochondrial (and other) membrane.

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Author notes

  1. P. Veit

    Present address: Gustav-Embden-Zentrum der Biologischen Chemie, Universität Frankfurt am Main, 6000, Frankfurt/M., (F.R.G.)

Authors and Affiliations

  1. Gustav-Embden-Zentrum der Biologischen Chemie, Universität Frankfurt am Main, 6000, Frankfurt/M., (F.R.G.)

    J. Fuchs & G. Zimmer

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  1. P. Veit
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  2. J. Fuchs
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  3. G. Zimmer
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Additional information

This work was supported by the Deutsche Forschungsgemeinschaft, Zi 80/17

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Veit, P., Fuchs, J. & Zimmer, G. Uncoupler-and hypoxia-induced damage in the working rat heart and its treatment. Basic Res Cardiol 80, 107–115 (1985). https://doi.org/10.1007/BF01910457

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  • DOI: https://doi.org/10.1007/BF01910457

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