Summary
Successive ischemic episodes are commonly seen in clinical and experimental cardiology. Although prolonged abnormalities of the canine myocardium have been described following a single ischemic episode, it is not known whether myocardial injury is cumulative following multiple ischemic episodes. Dogs were subjected to three 15-min left anterior descending coronary artery occlusions, each followed by 30 min of reperfusion.In vivo biopsies were obtained for biochemical analysis before and during the first occlusion and 30 min into each reperfusion period. ATP and creatine phosphate (CP) fell in the endocardium during occlusion by 24% and by 69% respectively (both p<.0001). While CP recovered during each reperfusion period, ATP remained significantly depressed. Loss of membrane-permeable purine nucleotide synthesis precursors occurred with the first reperfusion period but not with the second and third reperfusion periods. Therefore, reperfusion following one 15-min coronary occlusion is associated with severe depression of myocardial high energy phosphates; however, two additional occlusions do not cause a further decrease of these substances when intermittent reperfusion is allowed for 30 min.
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Supported in part by Grants HL 23140, 28048 and SCOR 26215 under the National Heart, Lung and Blood Institute, Bethesda, Maryland
This work was done during the tenure of Drs. Kloner and Ingwall as Established Investigators from the American Heart Association and with funds contributed in part by the Massachusetts Heart Association.
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Lange, R., Ingwall, J.S., Hale, S.L. et al. Effects of recurrent ischemia on myocardial high energy phosphate content in canine hearts. Basic Res Cardiol 79, 469–478 (1984). https://doi.org/10.1007/BF01908148
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DOI: https://doi.org/10.1007/BF01908148