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Reduced number of digitalis receptor sites in the hypertrophied pig myocardium

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Summary

Left ventricular hypertrophy was established in young pigs (35–40 kg) after 4 weeks of supravalvular aortic stenosis. Isolated sarcolemmal membrane fraction was characterized for specific3H-ouabain binding. Analysis revealed a single class of receptor sites of 5.7 p moles/mg protein and a dissociation constant (Kd) of the order of 6.25×10−8 M. Measurement of specific3H-ouabain binding with sarcolemmal fraction isolated from hypertrophied heart revealed a 26% reduction of digitalis receptor after 4 weeks of aortic stenosis, without any significant change in the affinity of receptor sites. This change in the number of digitalis receptors was maintained between 4 and 8 weeks of aortic stenosis and may be an important adaptive mechanism.

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Author notes

  1. J. C. Khatter

    Present address: Department of Medicine, C4, Health Sciences Centre, 700 William Avenue, R3E OZ3, Winnipeg, Manitoba, Canada

Authors and Affiliations

  1. Department of Medicine, Section of Cardiology, University of Manitoba, Winnipeg, Canada

    J. C. Khatter & R. J. Hoeschen

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  1. J. C. Khatter
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  2. R. J. Hoeschen
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This investigation was supported by a grant from the Medical Research Council of Canada

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Khatter, J.C., Hoeschen, R.J. Reduced number of digitalis receptor sites in the hypertrophied pig myocardium. Basic Res Cardiol 79, 396–401 (1984). https://doi.org/10.1007/BF01908139

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  • DOI: https://doi.org/10.1007/BF01908139

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