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Myocardial cAMP and calcium levels in the calcium paradox

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Summary

In a graded model (minimal, subtotal, total) of the calcium paradox phenomenon induced by a progressive increase in the flow rate and volume (5 ml, 10 ml, 45 ml) of calcium-free perfusion (5 min) the severity of tissue injury on calcium repletion was related to a potential elevation of cAMP during calcium depletion. In the subtotal and total models of the calcium paradox a 50% increase was found for tissue cAMP after calcium-free perfusion, but no such rise could be associated with the minimal calcium paradox. In the study tissue injury, as assessed by whole tissue and mitochondrial accumulation of calcium and by myocardial leakage of creatine kinase, could only partly be related to cAMP changes. It is concluded that calcium-free coronary perfusion induces a complex series of events favouring excessive calcium entry, only one of which may be related to a change in cAMP.

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Author notes

  1. S. Rotevatn

    Present address: Department of Anatomy, University of Bergen, Årstadvn. 19, N-5000, Bergen, Norway

Authors and Affiliations

  1. Department of Pharmacology and Toxicology Faculty of Medicine, University of Trondheim, Trondheim, (Norway)

    A. N. Øksendal & P. Jynge

  2. Department of Anatomy, University of Bergen, Årstadvn. 19, N-5000, Bergen, Norway

    H. Jodalen, J. Røli & D. Øgreid

Authors
  1. S. Rotevatn
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  2. H. Jodalen
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  3. J. Røli
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  4. D. Øgreid
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  5. A. N. Øksendal
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  6. P. Jynge
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Additional information

This work was supported by grants from the Norwegian Research Council on Cardiovascular Disease and from The Norwegian Research Council for Science and the Humanites.

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Rotevatn, S., Jodalen, H., Røli, J. et al. Myocardial cAMP and calcium levels in the calcium paradox. Basic Res Cardiol 81, 430–435 (1986). https://doi.org/10.1007/BF01907464

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  • DOI: https://doi.org/10.1007/BF01907464

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