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The effect of hypothermia during the period of calcium repletion on the calcium paradox

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Summary

Reperfusion of an isolated heart with a calcium-containing solution after a short calcium-free perfusion may result in irreversible cell damage: the calcium paradox. In this investigation the effect of hypothermia during reperfusion with calcium-containing solution on the calcium paradox damage in the isolated rat heart was studied. In addition, the effect of pre-cooling the heart during the calcium-free period was investigated. Creatine kinase release was used to define cell damage. Normothermic (37°C) calcium-free perfusion followed by normothermic reperfusion with calcium-containing solution resulted in a massive release of CK. When the normothermic calcium-free perfusion was followed by hypothermic (10°C) calcium-containing reperfusion, CK release was reduced by 20% (P<0.005). This CK release during reperfusion was further reduced by 55% and 80% when the normothermic calcium-free perfusion was followed by 5 or 10 min respectively of hypothermic calcium-free perfusion prior to the hypothermic calcium-containing reperfusion. The results show that hypothermia during the period of calcium repletion retards the sequence of events which ultimately results in release of large amounts of intracellular components.

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Author notes

  1. T. J. C. Ruigrok

    Present address: Department of Cardiology, University Hospital, Catharijnesingel 101, 3511 GV Utrecht, The Netherlands

Authors and Affiliations

  1. Department of Cardiology, University Hospital, Catharijnesingel 101, 3511 GV Utrecht, The Netherlands

    D. de Moes & P. van der Meer

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  1. T. J. C. Ruigrok
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  2. D. de Moes
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  3. P. van der Meer
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Ruigrok, T.J.C., de Moes, D. & van der Meer, P. The effect of hypothermia during the period of calcium repletion on the calcium paradox. Basic Res Cardiol 81, 425–429 (1986). https://doi.org/10.1007/BF01907463

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  • DOI: https://doi.org/10.1007/BF01907463

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