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Effect of tetrodotoxin, lidocaine, and quinidine on the transient inward current of sheep Purkinje fibres

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Summary

The effect of tetrodotoxin (TTX), lidocaine, and quinidine on the transient inward current (TI) was studied in voltage-clamped sheep cardiac Purkinje fibres. The TI was induced by elevation of extracellular Ca or addition of strophanthidin.

Reduction of external Na had a biphasic effect on the steady state TI magnitude; a moderate (<50%) reduction of external Na had an enhancing effect on the TI; a further decrease of extracellular Na was accompanied by a decline of TI amplitude. The TI could not be induced in Na-free medium (external Ca≦9.0 mM).

TTX, lidocainc, and quinidine reduced the magnitude of the TI in a dose-dependent way. The blocking effect of these agents could be compensated for by a moderate (<50%) reduction of external Na or an elevation of extracellular Ca.

It is suggested that the blocking effect of TTX, lidocaine, and quinidine on the TI is due to a reduction of intracellular Na, which causes a decay of intracellular Ca via the Na−Ca exchange mechanism.

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Author notes

  1. B. Henning

    Present address: Medizinische Klinik, Abt. Kardiologie, Universität Freiburg, Hugstetter Straße 55, 7800, Freiburg, FRG

Authors and Affiliations

  1. Medizinische Klinik, Abt. Kardiologie, Universität Freiburg, Hugstetter Straße 55, 7800, Freiburg, FRG

    M. Zehender, T. Meinertz & H. Just

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Henning, B., Zehender, M., Meinertz, T. et al. Effect of tetrodotoxin, lidocaine, and quinidine on the transient inward current of sheep Purkinje fibres. Basic Res Cardiol 83, 176–189 (1988). https://doi.org/10.1007/BF01907272

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  • DOI: https://doi.org/10.1007/BF01907272

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