Summary
-
1.
The cell envelope of frozen-etchedN. europaea cells showed a smooth outer boundary
revealed by etching. Splitting of the outer cell envelope occurred frequently and resulted in the appearance of a smooth convex fracture face
presumably corresponding to a concave fracture face
showing a dense arrangement of subunits.
-
2.
The convex fracture faces of the cytoplasmic membrane and intracytoplasmic membranes were very similar. The intracytoplasmic membranes covered the entire cell length and became constricted in dividing cells. Their precise arrangement depended on the conditions in which the cells had been frozen.
Similar content being viewed by others
References
De Voe, I. W., Costerton, J. W., Mac Leod, R. A.: Demonstration by freeze-etching of a single cleavage plane in the cell wall of a gram negative bacterium. J. Bact.106, 659–671 (1971).
Fiil, A., Branton, D.: Changes in the plasma membrane ofEscherichia coli during magnesium starvation. J. Bact.98, 1320–1327 (1969).
Murray, R. G. E., Watson, S. W.: Structure ofNitrosocystis oceanus and comparison withNitrosomonas andNitrobacter. J. Bact89, 1594–1609 (1965).
Nanninga, N.: Ultrastructure of the cell envelope ofEscherichia coli B after freeze-etching. J. Bact.101, 297–303 (1970).
—: Uniqueness and location of the fracture plane in the plasma membrane ofBacillus subtilis. J. Cell Biol.49, 564–570 (1971).
Remsen, C. C., Lundgren, D. G.: Electron microscopy of the cell envelope ofFerrobacillus ferrooxidans prepared by freeze-etching and chemical fixation technique. J. Bact.92, 1765–1771 (1966).
—, Valois, F. M., Watson, S. W.: Fine structure of the cytomembranes ofNitrosocystis oceanus. J. Bact.94, 422–433 (1967).
Sleytr, U. B.: Fracture faces in intact cells and protoplasts ofBacillus stearothermophilus. A study by conventional freeze-etching of corresponding fracture moieties. Protoplasma (Wien)71, 295–312 (1970).
Staehelin, L. A.: Chloroplast fibrils linking the photosynthetic lamellae. Nature (Lond.)214, 1158 (1967).
Tsien, H. C., Lambert, R., Laudelout, H.: Fine structure and the localization of the nitrite oxidizing system inNitrobacter winogradskyi. Antonie v. Leeuwenhoek34, 483–494 (1968).
Van Gool, A. P., Lambert, R., Laudelout, H.: The fine structure of frozen-etchedNitrobacter cells. Arch. Mikrobiol.69, 281–293 (1969).
—, Nanninga, N.: Fracture faces in the cell envelope ofEscherichia coli. J. Bact.108, 474–481 (1971).
Watson, S. W.: Reisolation ofNitrosospira briensis S. Winogradsky and H. Winogradsky 1933. Arch. Mikrobiol.75, 179–188 (1971).
—, Graham, L. B., Remsen, C. C., Valois, F. W.: A lobular ammonia oxidizing bacteriumNitrosolobus multiformis Nov. Gen. Nov. Sp. Arch. Mikrobiol.76, 183–203 (1971).
—, Remsen, C. C.: Cell envelope ofNitrosocystic oceanus. J. Ultrastruct. Res.33, 148–160 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van Gool, A.P. Ultrastructure ofNitrosomonas europaea cells as revealed by freeze-etching. Archiv. Mikrobiol. 82, 120–127 (1972). https://doi.org/10.1007/BF01890403
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01890403