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Diphenylhexatriene-phosphatidylcholine fluorescence in POPC vesicles: Comparison of the exponential-series and the maximum-entropy methods

  • Fluorescence of Membranes
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Abstract

We have investigated the time-resolved fluorescence of diphenylhexatriene (DPH) covalently linked to phosphatidylcholine (PC) in palmitoyloleoylglycerophosphocholine (POPC) vesicles with special consideration of the comparison of two methods for distributional lifetime analysis: the exponential-series method (ESM) and the maximum-entropy method (MEM). Generally, both methods were found to reveal equivalent results on high-quality data. Different are the shapes of the recovered distributions (symmetry and width) as well as the time effort for the numerical analysis.

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References

  1. D. R. James and W. R. Ware (1986)Chem. Phys. Lett. 126, 7–11.

    Google Scholar 

  2. A. K. Livesey and J.-C. Brochon (1987)Biophys. J. 52, 693–706.

    Google Scholar 

  3. G. Landl, T. Langthaler, H. W. Engl, and H. F. Kauffmann (1991)J. Comp. Phys. 95, 1–28.

    Google Scholar 

  4. E. T. Jaynes (1968).IEEE Trans SSC 4, 227–241.

    Google Scholar 

  5. J. R. Alcala, E. Gratton, and F. G. Prendergast (1987)Biophys. J. 51, 597–604.

    PubMed  Google Scholar 

  6. B. R. Lentz (1989)Biophys. J. 56, 723–733.

    PubMed  Google Scholar 

  7. C. Zannoni, A. Arcioni, and P. Cavatorta (1983)Chem. Phys. Lipids 32, 179–250.

    Google Scholar 

  8. E. Kalb, F. Paltauf, and A. Hermetter (1989)Biophys. J. 56, 1245–1253.

    PubMed  Google Scholar 

  9. J. M. H. Kremer, M. W. van der Esker, J. C. Patmamanoharan, and P. H. Wiersema (1977)Biochemistry 16, 3932–3935.

    PubMed  Google Scholar 

  10. E. Prenner, A. Hermetter, G. Landl, H. Stuetz, H. F. Kauffmann, and A. J. Kungl (1993)J. Phys. Chem. 97, 2788–2792.

    Google Scholar 

  11. G. Landl (1992)The Maximum Entropy Method: Convergence, Analysis and an Application from Physical Chemistry, VWGÖ, Wien.

    Google Scholar 

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Kungl, A., Anrather, D., Landl, G. et al. Diphenylhexatriene-phosphatidylcholine fluorescence in POPC vesicles: Comparison of the exponential-series and the maximum-entropy methods. J Fluoresc 4, 361–363 (1994). https://doi.org/10.1007/BF01881457

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  • DOI: https://doi.org/10.1007/BF01881457

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