Abstract
We have investigated the time-resolved fluorescence of diphenylhexatriene (DPH) covalently linked to phosphatidylcholine (PC) in palmitoyloleoylglycerophosphocholine (POPC) vesicles with special consideration of the comparison of two methods for distributional lifetime analysis: the exponential-series method (ESM) and the maximum-entropy method (MEM). Generally, both methods were found to reveal equivalent results on high-quality data. Different are the shapes of the recovered distributions (symmetry and width) as well as the time effort for the numerical analysis.
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D. R. James and W. R. Ware (1986)Chem. Phys. Lett. 126, 7–11.
A. K. Livesey and J.-C. Brochon (1987)Biophys. J. 52, 693–706.
G. Landl, T. Langthaler, H. W. Engl, and H. F. Kauffmann (1991)J. Comp. Phys. 95, 1–28.
E. T. Jaynes (1968).IEEE Trans SSC 4, 227–241.
J. R. Alcala, E. Gratton, and F. G. Prendergast (1987)Biophys. J. 51, 597–604.
B. R. Lentz (1989)Biophys. J. 56, 723–733.
C. Zannoni, A. Arcioni, and P. Cavatorta (1983)Chem. Phys. Lipids 32, 179–250.
E. Kalb, F. Paltauf, and A. Hermetter (1989)Biophys. J. 56, 1245–1253.
J. M. H. Kremer, M. W. van der Esker, J. C. Patmamanoharan, and P. H. Wiersema (1977)Biochemistry 16, 3932–3935.
E. Prenner, A. Hermetter, G. Landl, H. Stuetz, H. F. Kauffmann, and A. J. Kungl (1993)J. Phys. Chem. 97, 2788–2792.
G. Landl (1992)The Maximum Entropy Method: Convergence, Analysis and an Application from Physical Chemistry, VWGÖ, Wien.
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Kungl, A., Anrather, D., Landl, G. et al. Diphenylhexatriene-phosphatidylcholine fluorescence in POPC vesicles: Comparison of the exponential-series and the maximum-entropy methods. J Fluoresc 4, 361–363 (1994). https://doi.org/10.1007/BF01881457
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DOI: https://doi.org/10.1007/BF01881457