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Cy5-Labeled Phosphatidylcholine

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Abstract

Fluorescent lipid probes are widely used as tools for studying the properties of biological membranes and for studies of such biological functions of lipids as metabolic pathways and the molecular nature of diseases. In this report, we propose the new Cy5-labeled phosphatidylcholine (Cy5-PC) fluorescent lipid probe and describe its geometrical parameters, its synthesis, its spectral characteristics, and data on its mobility in a lipid membrane. Су5-PC is an excellent probe for bioimaging, because the intensity of its fluorescence retains a linear dependence up to high concentrations. However, the use of Су5-PC in studies of membrane dynamics is impossible, since the probe is insensitive even to the phase transition in a lipid membrane.

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Funding

This study was supported by the Ministry of Science and Education of the Russian Federation, project no. 0729-2020-0039 as a basic part of the state task.

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Authors and Affiliations

  1. Lobachevsky State University of Nizhny Novgorod, 603950, Nizhny Novgorod, Russia

    I. A. Gracheva, O. G. Zamyshlyaeva, E. S. Kudriashova & A. Y. Fedorov

  2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    D. S. Tretiakova, E. L. Vodovozova & I. A. Boldyrev

Authors
  1. I. A. Gracheva
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  2. D. S. Tretiakova
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  3. O. G. Zamyshlyaeva
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  4. E. S. Kudriashova
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  5. E. L. Vodovozova
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  6. A. Y. Fedorov
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  7. I. A. Boldyrev
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Corresponding author

Correspondence to I. A. Boldyrev.

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This article does not contain any studies involving human participants and animals performed by any of the authors.

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The authors declare that they have no conflicts of interest.

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Translated by L. Onoprienko

Abbreviations: BODIPY, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; Cy5, cyanine fluorophore, containing the polymethine chain form five С atoms; Cy5-PC, Cy5-labeled phosphatidylcholine; DMPC, 1,2-dimiristoyl-sn-glycerophosphocholine; NBD, 4-nitrobenzo-2-oxa-1,3-diazole; TMB-PC, (1,3,5,7-tetramethyl-bodipy)-labeled phosphatidylcholine.

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Gracheva, I.A., Tretiakova, D.S., Zamyshlyaeva, O.G. et al. Cy5-Labeled Phosphatidylcholine. Russ J Bioorg Chem 47, 1114–1117 (2021). https://doi.org/10.1134/S1068162021050265

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  • DOI: https://doi.org/10.1134/S1068162021050265

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