Abstract
A rapid and simple semiquantitative method for monitoring the attachment of DNA to liposomes is described. This technique is based on the measurement of resonance energy transfer between a membrane-embedded fluorescent pyrene lipid donor and DNA labeled with adriamycin as an acceptor.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
H. Zhang, N. Buckley, K. Gibson, and S. Spiegel (1990)J. Biol. Chem. 265, 76–81.
N. N. Desai and S. Spiegel (1991)Biochem. Biophys. Res. Commun. 181, 361–366.
H. Zhang, N. N. Desai, J. M. Murphey, and S. Spiegel (1990)J. Biol. Chem. 265, 21309–21316.
E. Crooke, C. E. Castuma, and A. Kornberg (1992)J. Biol. Chem. 285, 503–506.
N. Hutchinson and H. Weintraub (1985)Cell 43, 471–482.
P. R. Cook (1991)Cell 66, 627–635.
P. K. J. Kinnunen, M. Rytömaa, A. Köiv, J. Lehtonen, P. Mustonen, and A. Aro (1993)Chem. Phys. Lipids 66, 75–85.
P. Mustonen and P. K. J. Kinnunen (1991)J. Biol. Chem. 266, 6302–6307.
C. A. Frederick, L. D. Williams, G. Ughetto, G. A. van der Marel, J. H. van Boom, H. Rich, and A. H.-J. Wang (1990)Biochemistry 29, 2538–2549.
P. Mustonen and P. K. J. Kinnunen (1993)J. Biol. Chem. 268, 1074–1080.
A. Köiv, P. Mustonen, and P. K. J. Kinnunen (1994)Chem. Phys. Lipids 70, 1–10.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Köiv, A., Kinnunen, P.K.J. Measurement of the binding of DNA to liposomes by resonance energy transfer. J Fluoresc 4, 345–347 (1994). https://doi.org/10.1007/BF01881453
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01881453