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Induction of cytolytic activity of lymphocytes from carcinomatous pleural effusion by IL-2 and autologous tumor cells

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Biotherapy

Abstract

We established a cell line (STKM-1) from tumor cells obtained from carcinomatous pleural effusion of a gastric cancer patient. The lymphocytes separated from her peripheral blood or pleural effusion were cryopreserved and immunological experiments were performed after the establishment of the cell line. They were treated with IL-2 or with both IL-2 and mitomycin C (MMC)-treated autologous STKM-1 cells. The cytolytic activity against STKM-1 cells was elevated in lymphocytes cultured with IL-2, and was more prominently augmented in lymphocytes cultured with both IL-2 and MMC-treated STKM-1 cells. The elevation in cytolytic activity was more marked with pleural effusion lymphocytes than with the peripheral blood lymphocytes. The results suggest that the lymphocytes obtained from the pleural effusion would be an excellent source for adoptive immunotherapy.

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Abbreviations

IL-2:

interleukin-2

LAK:

lymphokine activated killer

MLTC:

mixed lymphocyte tumor cell culture

MMC:

mitomycin C

MoAbs:

monoclonal antibodies

TIL:

tumor infiltrating lymphocytes

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Authors and Affiliations

  1. Department of Surgery, Kanagawa Cancer Center, Nakaocho, Asahiku, 241, Yokohama

    Motonori Sairenji, Hisahiko Motohashi, Osamu Kobayashi, Kenzou Okada, Takashi Okamoto & Mamoru Tsukuda

  2. Clinical Research Institute, Kanagawa Cancer Center, Nakaocho, Asahi-ku, 241, Yokohama

    Shunsuke Yanoma

  3. Kihara Institute for Biological Research, Yokohama City University, Nakamura-cho, Minami-ku, 232, Yokohama

    Makoto Umeda

Authors
  1. Motonori Sairenji
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  2. Shunsuke Yanoma
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  3. Hisahiko Motohashi
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  4. Osamu Kobayashi
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  5. Kenzou Okada
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  6. Takashi Okamoto
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  7. Mamoru Tsukuda
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  8. Makoto Umeda
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Sairenji, M., Yanoma, S., Motohashi, H. et al. Induction of cytolytic activity of lymphocytes from carcinomatous pleural effusion by IL-2 and autologous tumor cells. Biotherapy 6, 283–290 (1993). https://doi.org/10.1007/BF01878358

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  • DOI: https://doi.org/10.1007/BF01878358

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