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Quantification of coenzyme F420 analogues from methanogenic bacteria by HPLC and fluorimetry

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Biotechnology Techniques

Summary

A quantitative assay for analogues of coenzyme F420 is presented. The assay combines separation of the coenzymes by binary reversed-phase high performance liquid chromatography with detection by fluorescence, yielding high specificity and sensitivity. Quantification is by calibration with a coenzyme F420 standard or by employing coenzyme F420 fragments as internal standards.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld, NL-6525 ED, Nijmegen, The Netherlands

    L G M Gorris, A K Kivaisi & H J M Op den Camp

Authors
  1. L G M Gorris
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  2. A K Kivaisi
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  3. H J M Op den Camp
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Gorris, L.G.M., Kivaisi, A.K. & Op den Camp, H.J.M. Quantification of coenzyme F420 analogues from methanogenic bacteria by HPLC and fluorimetry. Biotechnol Tech 3, 239–244 (1989). https://doi.org/10.1007/BF01876056

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  • DOI: https://doi.org/10.1007/BF01876056

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