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Mitochondrial Coenzyme Q10 Determination via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry

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Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1264))

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Abstract

Coenzyme Q10 (CoQ10) is an essential part of the mitochondrial respiratory chain. Here, we describe an accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of mitochondrial CoQ10 in isolated mitochondria. In the assay, mitochondrial suspensions are spiked with CoQ10-[2H6] internal standard, extracted with organic solvents, and CoQ10 quantified by LC-MS/MS using multiple reaction monitoring (MRM).

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Authors and Affiliations

  1. Laboratory Division HUSLAB, Helsinki University Central Hospital, Haartmaninkatu 2, Helsinki, 00029, Finland

    Outi Itkonen & Ursula Turpeinen

Authors
  1. Outi Itkonen
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  2. Ursula Turpeinen
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Correspondence to Outi Itkonen .

Editor information

Editors and Affiliations

  1. Midwestern University, Glendale, Arizona, USA

    Volkmar Weissig

  2. ISANH, Paris, France

    Marvin Edeas

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Itkonen, O., Turpeinen, U. (2015). Mitochondrial Coenzyme Q10 Determination via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1264. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2257-4_24

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  • DOI: https://doi.org/10.1007/978-1-4939-2257-4_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2256-7

  • Online ISBN: 978-1-4939-2257-4

  • eBook Packages: Springer Protocols

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