Summary
A spectrophotometric assay for cephalosporin C was established usingAlcaligenes faecalis as a test organism. The optimal pH range for the sensitivity of the assay was pH 6.7–7.4. The dose-response curve became linear after 4 h incubation with 15% (v/v) dosage. Deviations between spectrophotometric and chemical assays were within 6%. The cost of the spectrophotometric assay is about one-hundredth that of the chemical assay.
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References
Hewitt, W. (1977), Microbiological assay, Ch 4, New York: Academic Press.
Holm, K.H. (1972), Anal. Chem. 44, 795–799.
Kavanagh, F. (1963), Analytical microbiology, vol 1, Ch 4, New York: Academic Press.
Kavanagh, F. (1972), Analytical microbiology, vol 2, Ch 3 & 4, New York: Academic Press.
Lubbe, C., Shen, Y.Q., and Demain, A.L. (1986); Appl. Biochem. Biotechnol. 12, 31–35.
Miller, R.P.M. (1962), Antibiot. Chemother. 12, 689–693.
Pan, S.C. (1954), Anal. Chem. 26, 1438–1444.
Shaw, W.H.C. and Duncombe, R.E. (1963), Analyst 88, 694–701.
Tanaka, A., Itoh, N. and Fukui, S. (1982), Agric. Biol. Chem. 46, 129–134.
White, E.R. and Fox, M. (1982), J. Antibiot. 35, 1538–1546.
Zanca, D.M. and Martin, J.F. (1983), J. Antibiot. 36, 700–708.
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Khang, YH., Shankar, H. & Senatore, F. A simple and cost-effective spectrophotometric assay for cephalosporin C usingAlcaligenes faecalis . Biotechnol Tech 2, 57–62 (1988). https://doi.org/10.1007/BF01874209
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DOI: https://doi.org/10.1007/BF01874209