Summary
The inhibition of strontium transport from erythrocyte ghosts by internal calcium was investigated. When active strontium transport was measured in the presence of increasing levels of internal calcium it was found that the inhibition of strontium transport started at an internal calcium level of 0.3mm and was virtually complete when this concentration reached 1.0mm. It was also noted that calcium transport was virtually constant between concentrations of 0.3 and 1.0mm. This experiment indicated that calcium did not inhibit strontium transport by competing for the active site of the transport system. This inhibition was partially reversed by increasing the internal magnesium concentration from 1 to 4mm. A higher level of magnesium at the time of lysis and during incubation enhanced strontium transport. However, the inhibition remained noncompetitive with respect to calcium. Manganese was also found to support calcium and strontium transport. However, it could not reverse the inhibition of strontium transport by internal calcium at any concentration tested. In fact, manganese restored the inhibition of strontium transport by calcium in ghosts that were prepared and incubated in solutions that had high magnesium levels.
Similar content being viewed by others
References
Bond, C.H., 1972. Ligand induced conformation changes in the (Mg2++Ca2+) dependent ATPase of red cell membranes.Biochim. Biophys. Acta 288:423
Bond, C.H., Clough, L.L. 1973. A soluble activator of (Mg2++Ca2+) dependent ATPase in human red cell membranes.Biochim. Biophys. Acta 323:542
Cha, Y.N., Lee, K.S. 1976. Phosphorylation of the red blood cell membrane during the active transport of Ca2+.J. Gen. Physiol. 67:251
Cha, Y.N., Shin, B.C., Lee, K.S. 1971. Active uptake of Ca and Ca activated ATPase in red cell membranes.J. Gen. Physiol. 57:202
Duffy, M.J., Schwarz, V. 1973. Calcium binding by the erythrocyte membrane.Biochim. Biophys. Acta 330:294
Dunham, E.T., Glynn, I.M. 1961. Adenosine triphosphatase activity and the active movement of alkali metal ions.J. Physiol. (London) 159:274
Fiske, C.H., Subbarow, Y. 1925. The colorimetric determination of phosphorus.J. Biol. Chem. 66:375
Garrahan, P.J., Rega, A.F. 1978. Activation of partial reactions of the Ca2+ ATPase from human red cells by Mg2+ and ATP.Biochim. Biophys. Acta 513:59
Graham, J.M., Wallach, D.F.H. 1971. Protein conformational transitions in the erythrocyte membrane.Biochim. Biophys. Acta 241:180
Knauf, P.A., Proverbio, F., Hoffman, J.F. 1974. Electrophoretic separation of different phosphoproteins associated with Ca-ATPase and Na, K-ATPase in human red cell ghosts.J. Gen. Physiol. 63:324
Olson, E.J., Cazort, R.J. 1969. Active calcium and strontium transport in human erythrocyte ghosts.J. Gen. Physiol. 53:311
Olson, E.J., Cazort, R.J. 1974. Investigation of the accompaniment of calcium transport from human erythrocyte ghosts.J. Gen. Physiol. 63:590
Quist, E.E., Roufogalis, B.D. 1975. Calcium transport in human erythrocytes. Separation and reconstitution of high and low affinity (Mg+Ca)-ATPase activities in membranes prepared at low ionic strength.Arch. Biochem. Biophys. 168:240
Rega, A.F., Garrahan, P.J. 1975. Calcium ion-dependent phosphorylation of human erythrocyte membranes.J. Membrane Biol. 22:313
Scharff, O. 1978. Stimulating effects of monovalent cations of activator dissociated and activator associated states of Ca ATPase in human erythrocytes.Biochim. Biophys. Acta 512:309
Schatzmann, H.J. 1966. ATP dependent Ca extrustion from human red cells.Experientia 22:364
Schatzmann, H.J. 1973. Dependence on calcium concentration and stoichiometry of calcium pump in human red cells.J. Physiol. (London) 235:551
Schatzmann, H.J., Vincenzi, F.F. 1969. Calcium movements across the membrane of human red cells.J. Physiol. (London) 201:369
Triplett, R.B., Wingate, J.M., Carraway, K.L. 1972. Calcium effects on erythrocyte membrane proteins.Biochem. Biophys. Res. Commun. 49:1014
Trudeau, P.L., Frier, E.F. 1967. Determination of calcium in urine and serum by atomic absorption spectrophotometry.Clin. Chem. 13:101
Wins, P., Schoeffeniels, E. 1966. Properties of a Mg+Ca dependent ATPase.Biochim. Biophys. Acta 120:341
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olson, E.J. Inhibition of active strontium transport from erythrocyte ghosts by internal calcium: Evidence for a specificity controlling site. J. Membrain Biol. 48, 265–284 (1979). https://doi.org/10.1007/BF01872895
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01872895