Summary
In the presence of added orthophosphate (Pi) there is a period during which sarcoplasmic reticulum vesicles (SR) accumulate calcium at a constant rate. This constant rate is increased and is reached sooner when the added Pi concentration is increased. A double reciprocal plot of rate and Pi concentration gives a straight line.
The Pi concentration required for half-maximum rate of calcium accumulation is the same for SR preparations from red and white rabbit muscles, although the maximum rates are widely different. During the ageing of SR preparations the Pi concentration required for half-maximum rate increases, but the maximum rate remains the same.
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References
Barlogie, B., Hasselbach, W., Makinose, M. 1971. Activation of calcium effect by ADP and inorganic phosphate.Fed. Eur. Biochem. Soc. Lett. 12:267
Ebashi, S., Endo, M. 1964. Further studies on the calcium-binding activity of the relaxing factor.In: Biochemistry of Muscle Contraction. J. Gergely editor. p. 199. Little Brown, Boston
Hasselbach, W. 1964. Relaxing factor and the relaxation of muscleProgr. Biophys. Mol. Biol. 14:167
Ikemoto, N. 1975. Transport and inhibitory Ca2+ binding sites on the ATPase enzyme isolated from the sarcoplasmic reticulum.J. Biol. Chem. 250:7219
Lorand, L., Molnar, J. 1962. Biochemical control of relaxation in muscle systems.In: Muscle as a Tissue. K. Rodahl and S.M. Horvath, editors. p. 97. McGraw-Hill, New York
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265
Makinose, M. 1971. Calcium efflux dependent formation of ATP from ADP and orthophosphate by the membranes of the sarcoplasmic vesicles.Fed. Eur. Biochem. Soc. Lett. 12:269
Makinose, M. 1972. Phosphoprotein formation during osmo-chemical energy conversion in the membrane of the sarcoplasmic reticulum.Fed. Eur. Biochem. Soc. Lett. 25:113
Makinose, M., Hasselbach, W. 1965. Die Einfluß von Oxalat auf den Calcium-Transport isolierter Vesikel des sarkoplasmatischen Reticulum.Biochem. Z. 343:360
Makinose, M., Hasselbach, W. 1971. ATP synthesis by the reverse of the sarcoplasmic calcium pump.Fed. Eur. Biochem. Soc. Lett. 12:271
Martonosi, A., Donley, J., Halpin, R.A. 1968. Sarcoplasmic reticulum. III. The role of phospholipids in the adenosine triphosphatase activity and Ca++ transport.J. Biol. Chem. 243:61
Martonosi, A., Feretos, R. 1964. Sarcoplasmic reticulum. I. The uptake of Ca++ by sarcoplasmic reticulum fragments.J. Biol. Chem. 239:648
Meis, L. de, Carvalho, M. da G.C. 1974. Role of the Ca2+ concentration gradient in the adenosine 5′-triphosphate-inorganic phosphate exchange catalysed by sarcoplasmic reticulum.Biochemistry 13:5032
Meis, L. de, Sorenson, M.M. 1975. ATP↔Pi exchange and membrane phosphorylation in sarcoplasmic reticulum vesicles: Activation by silver in the absence of a Ca2+ concentration gradient.Biochemistry 14:2739
Mermier, P., Hasselbach, W. 1975. The effect of calcium and phosphate on the biphasic calcium uptake by the sarcoplasmic reticulum.Z. Naturforsch. 30c:777
Ogawa, Y. 1970. Some properties of fragmented frog sarcoplasmic reticulum with particular reference to its response to caffeine.J. Biochem. (Tokyo) 67:667
Panet, R., Selinger, Z. 1972. Synthesis of ATP coupled to Ca2+ release from sarcoplasmic reticulum vesicles.Biochim. Biophys. Acta 255:34
Sreter, F.A. 1969. Temperature, pH and seasonal dependence of Ca-uptake and ATPase activity of white and red muscle microsomes.Arch. Biochem. Biophys. 134:25
Weber, A., Herz, R., Reiss, I. 1964a. Role of calcium in contraction and relaxation of muscle.Fed. Proc. 23:896
Weber, A., Herz, R., Reiss, I. 1964b. The regulation of myofibrillar activity by calcium.Proc. R. Soc. London B 160:489
Weber, A., Herz, R., Reiss, I. 1966. Study of the kinetics of calcium transport by isolated fragmented sarcoplasmic reticulum.Biochem Z. 345:329
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Newbold, R.P., Tume, R.K. Effect of orthophosphate on the rate of calcium uptake by red and white muscle sarcoplasmic reticulum. J. Membrain Biol. 48, 205–213 (1979). https://doi.org/10.1007/BF01872891
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DOI: https://doi.org/10.1007/BF01872891