Dependence of photosensitivity of bileaflet lipid membranes upon the chlorophyll and carotenoid content

Summary

Bileaflet lipid membranes were formed from solutions containing lecithin, chlorophyll and carotene in various concentrations. If all the above components were present at sufficient concentrations the membranes were photosensitive; i.e., a photocurrent was produced if a redox potential gradient was present across the membranes. The presence of chlorophyll and carotene were essential for the photosensitivity of the membranes. Photoresponse could be elicited by illuminating the membrane with light which did not excite carotene. On the other hand, elimination of the part of the light spectrum which excites chlorophyll led to the abolition of the photoresponse. The findings of this study are consistent with the assumption that the excited chlorophyll chromophores allow electron exchange at the membrane-water interface while the presence of carotene allows electron movement across the “bulk” lipid membrane.