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Characteristics of manganese current and its comparison with currents carried by other divalent cations in snail soma membranes

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Summary

Characteristics of currents carried by Mn2+ and other divalent cations were studied in the isolated identified neuron in the circumesophageal ganglia ofHelix aspersa using a suction pipette technique which allows internal perfusion of the cell body and voltage clamp. Increases in [Mn2+] 0 induced not only saturation of the peak ofI Mn but also shifts theI–V relationships along the voltage axis to the more positive potentials. Internal perfusion with F−, which blocks Ca channels, depressedI Mn. Diltiazem, an organic Ca blocker, inhibitedI Mn over the entire range of theI–V relation without shifting the threshold and peak voltage of theI–V relation. Co2+, Ni2+, Cd2+ and La3+ also suppressedI Mn. Relative maximum peak currents of the divalent cations wereI Ba=I Sr>I Ca>I Mn=I Zn. Time constants for activation (τ m ) and inactivation (τ h ) of these cations were voltage dependent, and both time constants were greater in the sequence ofI Mn=I Zn>I Ba=I Sr>I Ca over the whole voltage range.

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Authors and Affiliations

  1. Department of Physiology, Kyushu University Faculty of Medicine, 812, Fukuoka, Japan

    N. Akaike

  2. Department of Pharmacology, Kumamoto University Medical School, 860, Kumamoto, Japan

    K. Nishi & Y. Oyama

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  1. N. Akaike
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  2. K. Nishi
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  3. Y. Oyama
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Akaike, N., Nishi, K. & Oyama, Y. Characteristics of manganese current and its comparison with currents carried by other divalent cations in snail soma membranes. J. Membrain Biol. 76, 289–297 (1983). https://doi.org/10.1007/BF01870371

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  • DOI: https://doi.org/10.1007/BF01870371

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