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Studies on chloride permeability of the skin ofLeptodactylus ocellatus: III. Na+ and Cl− effect on electrical phenomena

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Summary

During their flux through the skin of the frogLeptodactylus ocellatus, Na+ and Cl− interact with each other. This interaction gives rise to electrical phenomena which are studied in the present paper. The skin is mounted in Na2SO4 Ringer's with 115 mM Na+ on the inside, and a variety of outer solutions,. The osmolarity of all solutions is kept constant at 237.8 mosmol by adding sucrose. When the main anion used on the outside is SO =4 the electrical potential difference (Δψ) rises steadily with the concentration of sodium (Na+)o up to 87 mV, which is reached at about 20mm. Thereafter Δψ remains constant. When the main anion is Cl− it is observed that Δψ rises steadily with (NaCl)o with a slope similar to the curve obtained with SO =4 (37 mV per decade), but with a lower intercept attributed to an inward Cl pumping which is characteristic of this frog species. At 2–9 mM (NaCl)o a Cl-specific channel is activated. Further increases of (NaCl)o produce a decrease of Δψ. The specificity of the activation of this site by monovalent cations and its use by monovalent anions is also studied.

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Author notes

  1. C. A. Rotunno

    Present address: Centro de Investigación y de Estudios Avanzados del I.P.N., Apartado Postal 14-740, México 14, D.F., Mexico

Authors and Affiliations

  1. Department of Biophysics, Albert Einstein Center of Medical Research (CIMAE), Luis Viale 2831, Buenos Aires, Argentina

    E. Rodríguez. Boulan, M. V. Ques-von Petery, C. A. Rotunno & M. Cereijido

  2. Centro de Investigación y de Estudios Avanzados del I.P.N., Apartado Postal 14-740, México 14, D.F., Mexico

    E. Rodríguez. Boulan & M. V. Ques-von Petery

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  1. E. Rodríguez. Boulan
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  2. M. V. Ques-von Petery
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  3. C. A. Rotunno
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  4. M. Cereijido
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Rodríguez. Boulan, E., Ques-von Petery, M.V., Rotunno, C.A. et al. Studies on chloride permeability of the skin ofLeptodactylus ocellatus: III. Na+ and Cl− effect on electrical phenomena. J. Membrain Biol. 42, 345–356 (1978). https://doi.org/10.1007/BF01870355

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  • DOI: https://doi.org/10.1007/BF01870355

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