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Effect of calcium on the H+/K+ ATPase of hog gastric microsomes

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Summary

The K+-stimulated, ouabain-insensitive ATPase activity present in vesicles of microsomal fractions from hog gastric mucosa can be demonstrated in fresh preparations by adding Ca2+ (μM range) to the incubation medium. Ca2+ effect is similar but not additive to the effect of gramicidin or freezing. High Ca2+ concentrations (1 mM) produce an inhibotory effect on the K+-stimulated ATPase activity. This effect, is not seen in the presence of gramicidin. Calcium increases the magnitude of ATP-driven H+ uptake in vesicles exposed to K+ for periods of time up to 60 min. At longer times of exposure (120 min) the response does not differ from controls. It is concluded that Ca2+ at low concentrations (μm range) enhances the K+ permeability of the vesicular membrane. At higher concentrations (mm range), Ca2+ becomes inhibitory to the K+ permeability. A role for Ca2+ as a second messenger in stimulus-secretion coupling in the parietal cell is discussed.

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  1. Centro de Biofísica y Bioquímica, Instituto Venezolano, de Investigaciones Científicas (IVIC), Apartado 1827, Caracas, Venezuela

    Fulgencio Proverbio & Fabián Michelangeli

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  1. Fulgencio Proverbio
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  2. Fabián Michelangeli
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Proverbio, F., Michelangeli, F. Effect of calcium on the H+/K+ ATPase of hog gastric microsomes. J. Membrain Biol. 42, 301–315 (1978). https://doi.org/10.1007/BF01870352

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  • DOI: https://doi.org/10.1007/BF01870352

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