Summary
99.8% Deuterium oxide, as obtained commerically, has been shown to contain a contaminant which strongly inhibits calcium transport and binding by sarcoplasmic reticulum (S.R.) and the associated ATPase activity. The contaminant is removed by distillation of deuterium oxide. Calcium binding by S.R. is maximal at pH 6.5 whereas calcium transport (in the presence of oxalate) is maximal at a pH of 7.2 to 7.5. In the presence of deuterium oxide, these maxima are shifted to a pD of 7.2 and a pD of 7.5 to 8.0, respectively. The maximum binding and transport rates are not affected by the change from aqueous to deuterium oxide medium. The same phenomena are observed with the ATPase activity. In the presence of oxalate, calcium;magnesium ATPase is maximal at pH 7.2 and pD 8.0. The maximum rate is unchanged, however,
At pH 7.2 or higher, the amount of calcium which may be bound by S.R. remains constant with time. At lower pH, calcium initially bound is slowly displaced from the membrane with time. It has been reported that deuterium oxide inhibits excitation-contraction coupling. The results presented here indicate that S.R. is probably not the site of deuterium oxide inhibition, and raise the possibility that the measured inhibition is due to an impurity in the deuterium oxide.
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References
Barnes, T. C., Cunliffe, T., Warren, J. 1935. The heart rate in heavy water.Science 81:346
Besch, H. R., Jr., Schwartz, A. 1971. Initial calcium binding rates of canine cardiac relaxing system (sarcoplasmic reticulum fragments) determined by stopped-flow spectrophotometry.Biochem. Biophys. Res. Cummun. 45:286
Brandt, W. 1935. The effect of deuterium oxide on the action of the isolated surviving frog heart.Klin. Wschr. 14:1597
Carter, N. W., Rector, F. C., Campion, D. S., Seldin, D. W. 1967. Measurement of intracellular pH of skeletal muscle with pH-sensitive glass microelectrodes.J. Clin. Invest. 46:920
Ebashi, S., Endo, M. 1968. Calcium ion and muscle contraction.Prog. Biophys. Mol. Biol. 18:123
Glasoe, P. D., Long, F. A. 1960. Use of glass electrodes to measure acidities in deuterium oxide.J. Phys. Chem. 64:188
Gornall, A. G., Bardawill, C. J., David, M. M. 1949. Determination of serum proteins by means of the biuret reaction.J. Biol. Chem. 177:751
Hotta, K., Morales, M. F. 1960. Myosin B nucleoside triphosphatase in deuterium oxide.J. Biol. Chem. 235:61
Huxtable, R., Bressler, R. 1973. Determination of orthophosphate.Analyt. Biochem. 54:604
Huxtable, R., Bressler, R. 1973. Effect of taurine on a muscle intracellular membrane.Biochim. Biophys. Acta 323:573
Kaminer, B., Kimura, J. 1972. Deuterium oxide: Inhibition of calcium release in muscle.Science 176:406
Katz, A. M., Repke, D. I., Upshaw, J. E., Polascik, M. A. 1970. Characterization of dog cardiac microsomes. Use of zonal centrifugation to fractionate fragmented sarcoplasmic reticulum, (Na++K+)-activated ATPase and mitochondrial fragments.Biochim. Biophys. Acta 205:473
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265
Martonosi, A., Feretos, R. 1964. Sarcoplasmic reticulum. 1. The uptake of Ca2+ by sarcoplasmic reticulum fragments.J. Biol. Chem. 239:648
Nakamaru, Y., Schwartz, A. 1970. Possible control of intracellular calcium metabolism by [H+]: Sarcoplasmic reticulum of skeletal and cardiac muscle.Biochem. Biophys. Res. Commun. 41:830
Nakamaru, Y., Schwartz, A. 1972. The influence of hydrogen ion concentration on calcium binding and release by skeletal muscle sarcoplasmic reticulum.J. Gen. Physiol. 59:22
Quinn, P. J., Dawson, R. M. C. 1972. The pH dependence of calcium absorption onto anionic phospholipid monolayers.Chem. Phys. Lipids 8:1
Schwartz, A. 1971. Calcium and the sarcoplasmic reticulum.In: Calcium and the Heart. P. Harris and L. H. Opie, editors. p. 66. Academic Press Inc., London and New York
Sreter, F. A. 1969. Temperature, pH and seasonal dependence of Ca-uptake and ATPase activity of white and red muscle microsomes.Arch. Biochem. Biophys. 134:25
Verzar, F., Haffter, C. 1935. The effect of heavy water (deuterium oxide) on isolated organs.Pflug. Arch. Ges. Physiol. 236:714
Weber, A., Herz, R., Reiss, I. 1964. Role of calcium in contraction and relaxation of muscle.Fed. Proc. 23:896
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Huxtable, R., Bressler, R. The effect of deuterium ion concentration on the properties of sarcoplasmic reticulum. J. Membrain Biol. 17, 189–197 (1974). https://doi.org/10.1007/BF01870179
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DOI: https://doi.org/10.1007/BF01870179