Summary
The membrane potentials and resistances of L cells were measured using a standard electrophysiological technique. The values obtained in physiological media were around −15 mV and 37 MΩ, respectively. Almost all the large nondividing L cells (giant L cells) showed spontaneous oscillations of the membrane potential between around −15 and −40 mV. Application of an appropriate electrical or mechanical stimulus was also capable of eliciting responses but such were usually induced only once. The total membrane conductance increased significantly and in parallel with such a hyperpolarizing response. Cooling of the cells and application of metabolic inhibitors to the cells completely blocked the spontaneous oscillation despite the fact that the electrically induced hyperpolarizing response remained. Intracellular K+, Na+ and Cl− concentrations were measured by means of a flame photometer and a chloridometer, and the equilibrium potential for each ion was estimated.
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References
Burrows, R., Lamb, J.F. 1962. Sodium and potassium fluxes in cells cultured from chick embryo heart muscle.J. Physiol. (London) 162:510
Gallin, E.K., Wiederhold, M.L., Lipsky, P.E., Rosenthal, A.S. 1975. Spontaneous and induced membrane hyperpolarizations in macrophages.J. Cell. Physiol. 86:653
Lamb, J.F., MacKinnon, M.G.A. 1971a. Effect of ouabain and metabolic inhibitors on the Na and K movements and nucleotide contents of L cells.J. Physiol. (London) 213:665
Lamb, J.F., MacKinnon, M.G.A. 1971b. The membrane potential and permeabilities of the L cell membrane to Na, K and chloride.J. Physiol. (London) 213:683
Levine, S. 1960. Effect of manipulation on32P loss from tissue culture cells.Exp. Cell Res. 19:220
Minna, J., Nelson, P., Peacock, J., Glazer, D., Nirenberg, M. 1971. Genes for neuronal properties expressed in neuroblastoma x L cell hybrids.Proc. Nat. Acad. Sci. USA 68:238
Nelson, P.G., Peacock, J.H. 1972. Acetylcholine responses in L cells.Science 177:1005
Nelson, P.G., Peacock, J.H. 1973. Transmission of an active electrical response between fibroblasts (L cells) in cell culture.J. Gen. Physiol. 62:25
Nelson, P.G., Peacock, J.H., Minna, J. 1972. An active electrical response in fibroblasts.J. Gen. Physiol. 60:58
Okada, Y., Doida, Y., Irimajiri, A., Tsuchiya, W., Inouye, A. 1975. Abstract,XIV Jap. Congr. Biophys. Soc. (Osaka) 349 (in Japanese)
Okada, Y., Inouye, A. 1976. Studies on the origin of the tip potential of glass microelectrode.Biophys. Struct. Mech. 2:21
Okada, Y., Ogawa, M., Aoki, N., Izutsu, K. 1973. The effect of K+ on the membrane potential in HeLa cells.Biochim. Biophys. Acta 291:116
Okada, Y., Roy, G., Tsuchiya, W., Doida, Y., Inouye, A. 1977. Oscillations of membrane potential in L cells. II. Effect of monovalent ion concentrations and conductance changes associated with oscillations.J. Membrane Biol. 35:337
Okada, Y., Sato, T., Inouye, A. 1975. Effects of potassium ions and sodium ions on membrane potential of epithelial cells in rat duodenum.Biochim. Biophys. Acta 413:104
Quissell, D.O., Suttie, J.W. 1973. Effect of fluoride and other metabolic inhibitors on intracellular sodium and potassium concentrations in L cells.J. Cell. Physiol. 82:59
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Okada, Y., Doida, Y., Roy, G. et al. Oscillations of membrane potential in L cells. J. Membrain Biol. 35, 319–335 (1977). https://doi.org/10.1007/BF01869957
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DOI: https://doi.org/10.1007/BF01869957