Summary
This paper presents several equations derived from an asymmetrical nonconcentrative carrier mechanism for biological transport. These equations inter-relate the kinetic constants derived from different types of kinetic experiments, and act as criteria for the validity of the mechanism. Because they require a precise numeral relationship between rate constants, these equations can act as efficient discriminators for transport systems which are not mediated by a simple carrier mechanism, while they can provide presumptive evidence for a carrier mechanism when the observed kinetic constants do fulfill the predictions.
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Hoare, D.G. Transport across biological membranes: A rigorous test for the carrier hypothesis. J. Membrain Biol. 11, 169–176 (1973). https://doi.org/10.1007/BF01869819
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DOI: https://doi.org/10.1007/BF01869819