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Effects of adrenal steroids on Na transport in the lower intestine (Coprodeum) of the hen

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Summary

The influence of adrenal steroids on sodium transport in hen coprodeum was investigated by electrophysiological methods. Laying hens were maintained on low-NaCl diet (LS), or on high-NaCl diet (HS). HS hens were pretreated with aldosterone (128 μg/kg) or dexamethasone (1 mg/kg) before experiment. A group of LS hens received spironolactone (70 or 160 mg/kg, for three days). The effects of these dietary and hormonal manipulations on the amiloride-sensitive part of the short-circuit current were examined. This part is in excellent agreement with the net Na flux, and therefore a direct electrical measurement for Na transport. After depolarizing the basolateral membrane potential with a high K concentration, the apical Na permeability and the intracellular Na activity were investigated by currentvoltage relations for the different experimental conditions.

Plasma aldosterone concentrations (PA) were low in HS hens, dexamethasone-treated HS hens and spironolactonetreated LS hens (<70pm). In contrast LS hens and aldosteronetreated HS hens had a PA concentration of 596±70 and 583±172pm, respectively. LS diet (chronic stimulation) had the largest stimulatory effect on Na transport and apical Na permeability. Hormone-treated animals had three- to fourfold lower values. Spironolactone supply in LS hens decreased Na transport and apical Na permeability about 50%.

The results provide evidence that both mineralo- and glucocorticoids stimulate Na transport in this tissue by increasing the apical Na permeability. Quantitative differences between acute and chronic stimulation reveal a secondary slower adaptation in apical membrane properties.

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Authors and Affiliations

  1. Institut für Zoophysiologie, Universität Hohenheim, D-7000, Stuttgart 70, Germany

    W. Clauss, J. E. Dürr & D. Guth

  2. Department of Veterinary Physiology and Biochemistry, The Royal Veterinary and Agricultural University, DK-1870, Frederiksberg C, Denmark

    E. Skadhauge

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  1. W. Clauss
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  3. D. Guth
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  4. E. Skadhauge
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Clauss, W., Dürr, J.E., Guth, D. et al. Effects of adrenal steroids on Na transport in the lower intestine (Coprodeum) of the hen. J. Membrain Biol. 96, 141–152 (1987). https://doi.org/10.1007/BF01869240

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