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K+ influx components in ascites cells: The effects of agents interacting with the (Na++K+)-pump

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Summary

Several agents known to interact with the (Na++K+)-pump were tested for their effects on the components of steady-state K+ flux in ascites cells.86Rb+ was used as a tracer for K+, and influx was differentiated into a ouabain-inhibitable “pump” component, a Cl−-dependent and furosemide-sensitive “exchange” component, and a residual “leak” flux. All agents tested (ouabain, quercetin, oligomycin, phosphate) affected both the “pump” flux and the Cl−-linked flux. These findings suggest a linkage between the activity of the Na/K ATPase and the Cl−-dependent K+ exchange flux. In the discussion we point out that the mechanism of this linkage could be direct; e.g., Cl−-dependent exchange may represent a mode of operation of the Na/K ATPase. However, data from this and other systems tend to suggest an indirect linkage between the Na+ pump and a KCl symporter, perhaps via a change in the level of intracellular ATP.

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References

  1. Bakker-Grunwald, T. 1978. Effect of anions on potassium selfexchange in ascites tumor cells.Biochim. Biophys. Acta 513:292

    PubMed  Google Scholar 

  2. Bakker-Grunwald, T., Sinensky, M. 1979.Biochim. Biophys. Acta 558:296

    PubMed  Google Scholar 

  3. Brown, K.D., Lamb, J.F. 1978. An altered response of virally transformed 3T3 cells to ouabain.Biochim. Biophys. Acta 510:292

    PubMed  Google Scholar 

  4. Claret, M., Mazet, J.L. 1972. Ionic fluxes and permeabilities of cell membranes in rat liver.J. Physiol. (London) 223:279

    Google Scholar 

  5. Crane, R.K. 1977. The gradient hypothesis and other models of carrier-mediated, active transport.Rev. Physiol. Biochem. Pharmacol. 78:99

    PubMed  Google Scholar 

  6. Funder, J., Wieth, J.O. 1967. Effects of some monovalent anions on fluxes of Na and K, and on glucose metabolism of ouabain treated human red cells.Acta Physiol. Scand. 71:168

    PubMed  Google Scholar 

  7. Geck, P., Heinz, E., Pietrzyk, C., Pfeiffer, B. 1978. The effect of furosemide on the ouabain-insensitive K+ and Cl− movement in Ehrlich cells.In: Cell Membrane Receptors for Drugs and Hormones: A Multidisciplinary Approach. R.W. Straub and L. Bolis, editors. pp. 301–307. Raven Press, New York.

    Google Scholar 

  8. Geck, P., Jacobi, B.C., Pietrzyk, C., Pfeiffer, B., Heinz, E. 1979. K+−Cl−-cotransport in Ehrlich cells.Fed. Proc. 38:886

    Google Scholar 

  9. Glynn, I.M., Karlish, S.J.D. 1975. The sodium pump.Annu. Rev. Physiol. 37:13

    PubMed  Google Scholar 

  10. Glynn, I.M., Karlish, S.J.D., Cavieres, J., Ellory, J.C., Lew, V.L., Jørgensen, P.L. 1974. The effects of an antiserum to Na+,K+-ATpase on the ion-transporting and hydrolytic activities of the enzyme.Ann. N.Y. Acad. Sci. 242:357

    PubMed  Google Scholar 

  11. Hilden, S., Hokin, L.E. 1975. Active potassium transport coupled to active sodium transport in vesicles reconstituted from purified sodium and potassium ion-activated adenosine triphosphatase from the rectal gland ofSqualus acanthias.J. Biol. Chem. 250:6296

    PubMed  Google Scholar 

  12. Hodgkin, A.L., Katz, B. 1949. The effect of sodium ions on the electrical activity of the giant axon of the squid.J. Physiol. (London) 108:37

    Google Scholar 

  13. Hoffman, J.F., Kregenow, F.M. 1966. The characterization of new energy dependent cation transport processes in red blood cells.Ann. N.Y. Acad. Sci. 137:566

    PubMed  Google Scholar 

  14. Lamb, J.F., McCall, D. 1972. Effect of prolonged ouabain treatment on Na, K, Cl and Ca concentration and fluxes in cultured human cells.J. Physiol. (London) 225:599

    Google Scholar 

  15. Lang, D.R., Racker, E. 1974. Effects of quercetin andF 1 inhibitor on mitochondrial ATPase and energy-linked reactions in submitochondrial particles.Biochim. Biophys. Acta 333:180

    Google Scholar 

  16. Lardy, H.A., Ferguson, S.M. 1969. Oxidative phosphorylation in mitochondria.Annu. Rev. Biochem. 38:991

    PubMed  Google Scholar 

  17. Laris, P.C., Henius, G.V. 1979. The effect of glucose metabolism on the membrane potential and volume of Ehrlich ascites tumor cells.Fed. Proc. 38:886

    Google Scholar 

  18. McManus, T.J., Schmidt, W.F., III. 1978. Ion and co-ion transport in avian red cells.In: Membrane Transport Processes. Vol. 1, p. 79. J.F. Hoffman, editor. Raven Press, New York

    Google Scholar 

  19. Mills, B., Tupper, J.T. 1975. Cation permeability and ouabain-insensitive cation flux in the Ehrlich ascites tumor cell.J. Membrane Biol. 20:75

    Google Scholar 

  20. Schatzmann, H.J. 1953. Herzglykoside als Hemmstoffe für den aktiven Kalium- und Natriumtransport durch die Erythrocyten-membran.Helv. Physiol. Pharmacol. Acta 11:346

    PubMed  Google Scholar 

  21. Simons, T.J.B. 1974. Potassium: potassium exchange catalysed by the sodium pump in human red cells.J. Physiol. (London) 237:123

    Google Scholar 

  22. Stein, W.D. 1967.In: The movement of molecules across cell membranes. Academic Press, New York

    Google Scholar 

  23. Suolinna, E.-M., Buchsbaum, R.N., Racker, E. 1975. The effect of flavonoids on aerobic glycolysis, and growth of tumor cells.Cancer Res. 35:1865

    PubMed  Google Scholar 

  24. Tupper, J.T. 1975. Cation flux in the Ehrlich ascites tumor cell. Evidence for Na+-for-Na+ and K+-for-K+ exchange diffusion.Biochim. Biophys. Acta 394:586

    PubMed  Google Scholar 

  25. Ussing, H.H. 1949. Transport of ions across cellular membranes.Physiol. Rev. 29:127

    Google Scholar 

  26. Whittam, R., Wheeler, K.P., Blake, A. 1964. Oligomycin and active transport reactions in cell membranes.Nature (London) 203:720

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, University of Colorado Medical Center, 80262, Denver, Colorado

    Tilly Bakker-Grunwald, J. Scott Andrew & Margaret C. Neville

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  1. Tilly Bakker-Grunwald
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  2. J. Scott Andrew
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  3. Margaret C. Neville
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Bakker-Grunwald, T., Andrew, J.S. & Neville, M.C. K+ influx components in ascites cells: The effects of agents interacting with the (Na++K+)-pump. J. Membrain Biol. 52, 141–146 (1980). https://doi.org/10.1007/BF01869119

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  • DOI: https://doi.org/10.1007/BF01869119

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