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The complete rate equation, including the explicit dependence on Na+ ions, for the influx of α-aminoisobutyric acid into mouse brain slices

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Summary

The rate equation, including dependence on Na+-ion concentration for the influx of α-aminoisobutyric acid into mouse brain slices incubated in isotonic glucose medium at 37°C, isv=0.402S/{1.02(1+788/[Na+]2)+S}+0.0477S, wherev=influx in μmol/min, g final wet wt of slices; [Na+]=concentration of Na+ ions in medium, inmm; andS=concentration of α-aminoisobutyric acid in medium, inmm. This equation shows two kinetically independent, parallel pathways of concentrative uptake: one, saturable and dependent on Na+; the other, unsaturable and independent of Na+. Influx is independent of ionic strength, Cl− ionper se, and a moderate increase in tonicity. The binding of substrate to the saturable carrier depends on the Na+ concentration; the maximum capacity of this carrier does not. For transport, 2 Na+ ions must interact with each saturable transport site. This does not imply coupling between the flux of Na+ and the flux of α-aminoisobutyric acid.

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  1. New York State Institute for Basic Research in Mental Retardation, 10314, Staten Island, New York

    Stephen R. Cohen

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Cohen, S.R. The complete rate equation, including the explicit dependence on Na+ ions, for the influx of α-aminoisobutyric acid into mouse brain slices. J. Membrain Biol. 52, 95–105 (1980). https://doi.org/10.1007/BF01869114

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