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Tumor colony formation from human spontaneous tumors in a methylcellulose monolayer system

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Research in Experimental Medicine

Summary

A methylcellulose monolayer system is described that facilitates the “in vitro” cultures of human spontaneous tumor cells. The tumor samples were disaggregated mechanically and cultured in 0.9% methylcellulose with 30% fetal calf serum in Iscove's modified Dulbecco's medium. Eighty-five individual tumor samples with different histological types were seeded, 45 gave rise to tumor cell colonies. The plating efficiency ranged from 0.02% to 0.22%. Cytologic and cytochemical staining from aspirated cells revealed the same morphology of cells as the cells in the suspension. A flattening of colonies, as it has been described by others using methylcellulose monolayer, was not observed.

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Authors and Affiliations

  1. Medizinische Klinik, Albert-Ludwigs-Universität, Hugstetterstr. 55, D-7800, Freiburg, Federal Republic of Germany

    H. A. Neumann, G. W. Löhr & A. A. Fauser

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  1. H. A. Neumann
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  2. G. W. Löhr
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  3. A. A. Fauser
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Neumann, H.A., Löhr, G.W. & Fauser, A.A. Tumor colony formation from human spontaneous tumors in a methylcellulose monolayer system. Res. Exp. Med. 184, 137–143 (1984). https://doi.org/10.1007/BF01852387

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  • DOI: https://doi.org/10.1007/BF01852387

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